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Literature summary for 4.2.2.2 extracted from

  • Wang, H.; Guo, Y.; Lv, F.; Zhu, H.; Wu, S.; Jiang, Y.; Li, F.; Zhou, B.; Guo, W.; Zhang, T.
    The essential role of GhPEL gene, encoding a pectate lyase, in cell wall loosening by depolymerization of the de-esterified pectin during fiber elongation in cotton (2010), Plant Mol. Biol., 72, 397-406.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Gossypium hirsutum

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
calculated from amino acid sequence Gossypium hirsutum

Organism

Organism UniProt Comment Textmining
Gossypium hirsutum Q4JLV6 variant acc 7235
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA-agarose column chromatography Gossypium hirsutum

Source Tissue

Source Tissue Comment Organism Textmining
fiber
-
Gossypium hirsutum
-
leaf PEL transcripts are much lower in leaf compared to fibres Gossypium hirsutum
-
ovulum PEL transcripts are much lower in ovule compared to fibres Gossypium hirsutum
-
root PEL transcripts are much lower in roots compared to fibres Gossypium hirsutum
-
stem PEL transcripts are much lower in stem compared to fibres Gossypium hirsutum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
de-esterified pectin PEL can randomly catalyze the apha(1-4) linkages of de-esterified pectin by beta-elimination Gossypium hirsutum 4,5-unsaturated oligogalacturonates
-
?
polygalacturonate
-
Gossypium hirsutum unsaturated oligogalacturonides
-
?

Synonyms

Synonyms Comment Organism
Pel
-
Gossypium hirsutum

pI Value

Organism Comment pI Value Maximum pI Value
Gossypium hirsutum calculated from amino acid sequence
-
7.1

Expression

Organism Comment Expression
Gossypium hirsutum the PEL gene is preferentially expressed in fibers at 10 days-post anthesis up

General Information

General Information Comment Organism
physiological function PEL may play an important role in the process of normal fiber elongation in cotton Gossypium hirsutum