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Literature summary for 4.2.2.2 extracted from
- Enzymatic properties of two catalytic modules of Clostridium stercorarium pectate lyase Pel9A (2006), Biosci. Biotechnol. Biochem., 70, 667-671.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| full-length enzyme and catalytic modules CM9-1 and CM9-2 are separately expressed in Escherichia coli | Thermoclostridium stercorarium |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | absolute requirement, maximal activity of recombinantly expressed full-length enzyme at 0.05 mM, maximal activity of recombinantly expressed catalytic module CM9-1 at 0.02 mM, maximal activity of recombinantly expressed catalytic module CM9-2 at 0.1 mM | Thermoclostridium stercorarium |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58513 | - |
x * 58513, recombinantly expressed catalytic module CM9-1, SDS-PAGE | Thermoclostridium stercorarium |
| 76383 | - |
x * 76383, recombinantly expressed catalytic module CM9-2, SDS-PAGE | Thermoclostridium stercorarium |
| 133297 | - |
x * 133297, recombinant full-length enzyme, SDS-PAGE | Thermoclostridium stercorarium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoclostridium stercorarium | Q76EC9 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectin | apple pectin, citrus pectin, as the percentage of methylation in pectin becomes higher, the activities become lower | Thermoclostridium stercorarium | ? | - |
? | |
| polygalacturonic acid | - |
Thermoclostridium stercorarium | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 133297, recombinant full-length enzyme, SDS-PAGE | Thermoclostridium stercorarium |
| ? | x * 58513, recombinantly expressed catalytic module CM9-1, SDS-PAGE | Thermoclostridium stercorarium |
| ? | x * 76383, recombinantly expressed catalytic module CM9-2, SDS-PAGE | Thermoclostridium stercorarium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Pel9A | - |
Thermoclostridium stercorarium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Thermoclostridium stercorarium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
pH 7, 10 min, absence of substrate, stable | Thermoclostridium stercorarium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Thermoclostridium stercorarium |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 10 | 4°C, 12 h, stable | Thermoclostridium stercorarium |
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