Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Thermoanaerobacter italicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km for citrus pectin: 5.0 g/l | Thermoanaerobacter italicus | |
additional information | - |
additional information | Km for pectate: 0.5 g/l | Thermoanaerobacter italicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stimulates | Thermoanaerobacter italicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
pectate lyase b shows two bands in non-denaturing PAGE: 200000 Da and 252000 Da | Thermoanaerobacter italicus |
93000 | - |
1 * 93000 + 1 * 158000, pectate lyase b, SDS-PAGE | Thermoanaerobacter italicus |
135000 | - |
1 * 135000, pectate lyase a, SDS-PAGE | Thermoanaerobacter italicus |
148000 | - |
pectate lyase a, non-denaturing PAGE | Thermoanaerobacter italicus |
158000 | - |
1 * 93000 + 1 * 158000, pectate lyase b, SDS-PAGE | Thermoanaerobacter italicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoanaerobacter italicus | - |
nov., pectate lyase a and pectate lyase b | - |
Purification (Comment) | Organism |
---|---|
pectate lyase a and pectate lyase b | Thermoanaerobacter italicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pectate | - |
Thermoanaerobacter italicus | ? | - |
? | |
pectin | - |
Thermoanaerobacter italicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 93000 + 1 * 158000, pectate lyase b, SDS-PAGE | Thermoanaerobacter italicus |
monomer | 1 * 135000, pectate lyase a, SDS-PAGE | Thermoanaerobacter italicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
pectate lyase a | Thermoanaerobacter italicus |
80 | - |
pectate lyase b | Thermoanaerobacter italicus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 85 | 60°C: about 55% of maximal activity, 80°C: about 90% of maximal activity, 85°C: about 20% of maximal activity, pectate lyase a | Thermoanaerobacter italicus |
60 | 90 | 60°C: about 45% of maximal activity, 90°C: about 30% of maximal activity, pectate lyase b | Thermoanaerobacter italicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
2 h, about 10% loss of activity | Thermoanaerobacter italicus |
80 | - |
half-life: about 60 min | Thermoanaerobacter italicus |
90 | - |
complete loss of activity after 5 min | Thermoanaerobacter italicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
pectate lyase a | Thermoanaerobacter italicus |
9 | - |
pectate lyase B | Thermoanaerobacter italicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | pH 6.0: about 40% of maximal activity, pH 10.0: about 50% of maximal activity, pectate lyase a | Thermoanaerobacter italicus |
7 | 10 | pH 7.0: about 35% of maximal activity, pH 10.0: 50% of maximal activity, pectate lyase b | Thermoanaerobacter italicus |