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Literature summary for 4.2.2.18 extracted from

  • Haraguchi, K.; Yoshida, M.; Ohtsubo, K.i.
    Thermostable inulin fructotransferase (DFA III-producing) from Arthrobacter sp. L68-1 (2005), Carbohydr. Polym., 59, 411-416.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
10
-
inulin pH 5.5, 55°C Arthrobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
2 * 43000, SDS-PAGE Arthrobacter sp.
73000
-
gel filtration Arthrobacter sp.

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
L68-1
-
Arthrobacter sp. L68-1
-
L68-1
-

Purification (Commentary)

Purification (Comment) Organism
-
Arthrobacter sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture supernatant
-
Arthrobacter sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
933
-
-
Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inulin
-
Arthrobacter sp. di-D-fructofuranose 1,2':2,1' dianhydride + ?
-
?
inulin
-
Arthrobacter sp. L68-1 di-D-fructofuranose 1,2':2,1' dianhydride + ?
-
?

Subunits

Subunits Comment Organism
? 2 * 43000, SDS-PAGE Arthrobacter sp.

Synonyms

Synonyms Comment Organism
inulin fructotransferase (DFA III-producing)
-
Arthrobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Arthrobacter sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 30°C: about 30% of maximal activity, 70°C: about 60% of maximal activity Arthrobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
1 h, pH 5.5, stable up to Arthrobacter sp.
85
-
1 h, pH 5.5, about 90% loss of activity Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6
-
Arthrobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
4 8 pH 4.0: about 40% of maximal activity, pH 8.0: about 50% of maximal activity Arthrobacter sp.