Cloned (Comment) | Organism |
---|---|
gene gaaB, DNA and amino acid sequence determination and analysis, recombinant expression in Saccharomyces cerevisiae | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Aspergillus niger | |
1.89 | - |
L-fuconate | recombinant enzyme, pH 7.0, temperature not specified in the publication | Aspergillus niger | |
3.92 | - |
L-galactonate | recombinant enzyme, pH 7.0, temperature not specified in the publication | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | G3Y683 | - |
- |
Aspergillus niger ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL 328 / USDA 3528.7 | G3Y683 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-gluconate | - |
Aspergillus niger | ? | - |
? | |
D-gluconate | - |
Aspergillus niger ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL 328 / USDA 3528.7 | ? | - |
? | |
D-Glucuronate | - |
Aspergillus niger | ? | - |
? | |
D-Glucuronate | - |
Aspergillus niger ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL 328 / USDA 3528.7 | ? | - |
? | |
D-mannonate | - |
Aspergillus niger | 2-dehydro-3-deoxy-D-mannonate + H2O | - |
? | |
L-Fuconate | - |
Aspergillus niger | ? | - |
? | |
L-Fuconate | - |
Aspergillus niger ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL 328 / USDA 3528.7 | ? | - |
? | |
L-fuconate + H2O | highly preferred enzyme | Aspergillus niger | 2-dehydro-3-deoxy-L-fuconate | - |
? | |
L-galactonate | - |
Aspergillus niger | 2-dehydro-3-deoxy-L-galactonate + H2O | - |
? | |
L-galactonate | - |
Aspergillus niger ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL 328 / USDA 3528.7 | 2-dehydro-3-deoxy-L-galactonate + H2O | - |
? | |
L-mannonate | - |
Aspergillus niger | 2-dehydro-3-deoxy-L-mannonate + H2O | - |
? | |
additional information | the L-galactonate dehydratase shows highest activity with L-fuconate. The production of 2-dehydro-3-deoxy sugar is measured with the thiobarbituric acid assay. Substrate specificity analysis, overview. No activity with L-rhamnonate, D-gulonate, L-gulonate, D-arabonate, D-lyxonate, L-lyxonate, D-xylonate, mucic acid, D-riborate, L-arabonate, D-galactonate, and D-galacturonate | Aspergillus niger | ? | - |
? | |
additional information | the L-galactonate dehydratase shows highest activity with L-fuconate. The production of 2-dehydro-3-deoxy sugar is measured with the thiobarbituric acid assay. Substrate specificity analysis, overview. No activity with L-rhamnonate, D-gulonate, L-gulonate, D-arabonate, D-lyxonate, L-lyxonate, D-xylonate, mucic acid, D-riborate, L-arabonate, D-galactonate, and D-galacturonate | Aspergillus niger ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL 328 / USDA 3528.7 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
gaaB | - |
Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Aspergillus niger |