General Stability | Organism |
---|---|
glycerol stabilizes the apoenzyme | Klebsiella pneumoniae |
K2SO3 stabilizes the holoenzyme | Klebsiella pneumoniae |
stabilization by substrate 1,3-butanediol | Klebsiella pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
tetraethylammonium chloride | blocks the apoenzyme assay | Klebsiella pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Klebsiella pneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | required | Klebsiella pneumoniae | |
K+ | required for holoenzyme | Klebsiella pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
gel filtration, in the presence of ethanolamine buffer containing 0.1 M KCl, subunit B dissociates into sub-subunits | Klebsiella pneumoniae |
189000 | - |
gel filtration, in the presence of ethanolamine buffer, subunit B | Klebsiella pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerol | Klebsiella pneumoniae | - |
? | - |
? | |
glycerol | Lactobacillus leichmannii | - |
? | - |
? | |
glycerol | Klebsiella pneumoniae PZH 572 Warsaw | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
- |
- |
Klebsiella pneumoniae PZH 572 Warsaw | - |
- |
- |
Lactobacillus leichmannii | - |
- |
- |
Oxidation Stability | Organism |
---|---|
under aerobic conditions of incubation holoenzyme is quite unstable and quickly undergoes spontaneous irreversible inactivation | Klebsiella pneumoniae |
Purification (Comment) | Organism |
---|---|
apoenzyme, AB | Klebsiella pneumoniae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,3-butanediol | - |
Klebsiella pneumoniae | ? + H2O | - |
? | |
1,3-butanediol | - |
Klebsiella pneumoniae PZH 572 Warsaw | ? + H2O | - |
? | |
glycerol | - |
Klebsiella pneumoniae | 3-hydroxypropanal + H2O | beta-hydroxypropionaldehyde | ? | |
glycerol | - |
Lactobacillus leichmannii | 3-hydroxypropanal + H2O | beta-hydroxypropionaldehyde | ? | |
glycerol | - |
Klebsiella pneumoniae PZH 572 Warsaw | 3-hydroxypropanal + H2O | beta-hydroxypropionaldehyde | ? | |
glycerol | - |
Klebsiella pneumoniae | ? | - |
? | |
glycerol | - |
Lactobacillus leichmannii | ? | - |
? | |
glycerol | - |
Klebsiella pneumoniae PZH 572 Warsaw | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
30 min, potassium phosphate buffer, pH 8.6, apoenzyme is quite stable. On addition of coenzyme B12 to the incubation mixture the holoenzyme is formed | Klebsiella pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
5,6-Dimethylbenzimidazolylcobamide | coenzyme required | Klebsiella pneumoniae | |
5,6-Dimethylbenzimidazolylcobamide | coenzyme required | Lactobacillus leichmannii |