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Literature summary for 4.2.1.20 extracted from

  • Murciano-Calles, J.; Romney, D.; Brinkmann-Chen, S.; Buller, A.; Arnold, F.
    A Panel of TrpB biocatalysts derived from tryptophan synthase through the transfer of mutations that mimic allosteric activation (2016), Angew. Chem. Int. Ed. Engl., 55, 11577-11581 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.20

Cloned(Commentary)

Cloned (Comment) Organism
-
 Pyrococcus furiosus
-
 Escherichia coli
-
 Thermotoga maritima
-
 Archaeoglobus fulgidus

Protein Variants

Protein Variants Comment Organism
M144T/N166D 5fold increase in catalytic efficiency Pyrococcus furiosus
M145T/N167D mutant accepts various substrates with substitutions in position 5 of indole Thermotoga maritima
M149T/N171D 2fold increase in catalytic efficiency Escherichia coli
M156T/N178D 5fold increase in catalytic efficiency Archaeoglobus fulgidus
P12L/E17G/I68V/F274S/T292S/T321A 9fold increase in kcat with an equivalent decrease in KM Pyrococcus furiosus
P14L/P19G/I69V/L274S/T292S 2fold decrease in catalytic efficiency Thermotoga maritima
P19G/I69V/T292S 10fold increase in catalytic efficiency Thermotoga maritima
P25L/P30G/I80V/L285S/T303S/T321A 18fold increase in catalytic efficiency Archaeoglobus fulgidus
T292S 7fold increase in catalytic efficiency Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
 indole mutant P12L/E17G/I68V/F274S/T292S/T321A, pH 8.0, 75°C Pyrococcus furiosus
0.011
-
 indole mutant M156T/N178D, pH 8.0, 60°C Archaeoglobus fulgidus
0.012
-
 indole wild-type, pH 8.0, 60°C Archaeoglobus fulgidus
0.018
-
 indole mutant M149T/N171D, pH 8.0, 37°C Escherichia coli
0.019
-
 indole wild-type, pH 8.0, 37°C Escherichia coli
0.025
-
 indole mutant T292S, pH 8.0, 75°C Thermotoga maritima
0.026
-
 indole mutant P19G/I69V/T292S, pH 8.0, 75°C Thermotoga maritima
0.032
-
 indole mutant M145T/N167D, pH 8.0, 75°C Thermotoga maritima
0.033
-
 indole wild-type, pH 8.0, 75°C Thermotoga maritima
0.042
-
 indole mutant M144T/N166D, pH 8.0, 75°C Pyrococcus furiosus
0.048
-
 indole mutant P25L/P30G/I80V/L285S/T303S/T321A, pH 8.0, 60°C Archaeoglobus fulgidus
0.072
-
 indole mutant P14L/P19G/I69V/L274S/T292S, pH 8.0, 75°C Thermotoga maritima
0.077
-
 indole wild-type, pH 8.0, 75°C Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus O28672
-
-
Escherichia coli P0A879
-
-
Pyrococcus furiosus Q8U093
-
-
Thermotoga maritima P50909
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine + 1H-indole-5-carbaldehyde substrate of mutant M145T/N167D, 32% yield Thermotoga maritima 5-formyl-L-tryptophan + H2O
-
 ?
L-serine + 1H-indole-5-carbaldehyde substrate of mutant P12L/E17G/I68V/F274S/T292S/T321A Pyrococcus furiosus 5-formyl-L-tryptophan + H2O
-
 ?
L-serine + 5-bromoindole
-
 Pyrococcus furiosus 5-bromo-L-tryptophan + H2O
-
 ?
L-serine + 5-bromoindole substrate of mutant M145T/N167D, 25% yield Thermotoga maritima 5-bromo-L-tryptophan + H2O
-
 ?
L-serine + 5-bromoindole substrate of mutant P12L/E17G/I68V/F274S/T292S/T321A Pyrococcus furiosus 5-bromo-L-tryptophan + H2O
-
 ?
L-serine + 5-chloroindole substrate of mutant M145T/N167D, 93% yield Thermotoga maritima 5-chloro-L-tryptophan + H2O
-
 ?
L-serine + 5-chloroindole substrate of mutant P12L/E17G/I68V/F274S/T292S/T321A Pyrococcus furiosus 5-chloro-L-tryptophan + H2O
-
 ?
L-serine + 5-cyanoindole substrate of mutant M145T/N167D, 49% yield Thermotoga maritima 5-cyano-L-tryptophan + H2O
-
 ?
L-serine + 5-hydroxyindole substrate of mutant M145T/N167D, 93% yield Thermotoga maritima 5-hydroxy-L-tryptophan + H2O
-
 ?
L-serine + 5-hydroxyindole substrate of mutant P12L/E17G/I68V/F274S/T292S/T321A Pyrococcus furiosus 5-hydroxy-L-tryptophan + H2O
-
 ?
L-serine + 5-methoxyindole substrate of mutant M145T/N167D, 76% yield Thermotoga maritima 5-methoxy-L-tryptophan + H2O
-
 ?
L-serine + 5-methoxyindole substrate of mutant P12L/E17G/I68V/F274S/T292S/T321A Pyrococcus furiosus 5-methoxy-L-tryptophan + H2O
-
 ?
L-serine + 5-methylindole substrate of mutant M145T/N167D, 91% yield Thermotoga maritima 5-methyl-L-tryptophan + H2O
-
 ?
L-serine + 5-methylindole substrate of mutant P12L/E17G/I68V/F274S/T292S/T321A Pyrococcus furiosus 5-methyl-L-tryptophan + H2O
-
 ?
L-serine + 5-nitroindole substrate of mutant M145T/N167D, 88% yield Thermotoga maritima 5-nitro-L-tryptophan + H2O
-
 ?
L-serine + 5-nitroindole substrate of mutant P12L/E17G/I68V/F274S/T292S/T321A Pyrococcus furiosus 5-nitro-L-tryptophan + H2O
-
 ?
L-serine + indole
-
 Pyrococcus furiosus L-tryptophan + H2O
-
 ?
L-serine + indole
-
 Escherichia coli L-tryptophan + H2O
-
 ?
L-serine + indole
-
 Thermotoga maritima L-tryptophan + H2O
-
 ?
L-serine + indole
-
 Archaeoglobus fulgidus L-tryptophan + H2O
-
 ?

Synonyms

Synonyms Comment Organism
trpB
-
 Escherichia coli
trpB
-
 Thermotoga maritima
trpB
-
 Archaeoglobus fulgidus
TrpB1
-
 Pyrococcus furiosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.074
-
 indole wild-type, pH 8.0, 60°C Archaeoglobus fulgidus
0.11
-
 indole mutant P14L/P19G/I69V/L274S/T292S, pH 8.0, 75°C Thermotoga maritima
0.16
-
 indole wild-type, pH 8.0, 37°C Escherichia coli
0.31
-
 indole wild-type, pH 8.0, 75°C Pyrococcus furiosus
0.34
-
 indole mutant M149T/N171D, pH 8.0, 37°C Escherichia coli
0.34
-
 indole mutant M156T/N178D, pH 8.0, 60°C Archaeoglobus fulgidus
0.51
-
 indole mutant P25L/P30G/I80V/L285S/T303S/T321A, pH 8.0, 60°C Archaeoglobus fulgidus
0.83
-
 indole mutant M144T/N166D, pH 8.0, 75°C Pyrococcus furiosus
1.28
-
 indole wild-type, pH 8.0, 75°C Thermotoga maritima
2.9
-
 indole mutant P12L/E17G/I68V/F274S/T292S/T321A, pH 8.0, 75°C Pyrococcus furiosus
3.3
-
 indole mutant M145T/N167D, pH 8.0, 75°C Thermotoga maritima
5.8
-
 indole mutant T292S, pH 8.0, 75°C Thermotoga maritima
9.8
-
 indole mutant P19G/I69V/T292S, pH 8.0, 75°C Thermotoga maritima

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4
-
 indole wild-type, pH 8.0, 75°C Pyrococcus furiosus
6
-
 indole wild-type, pH 8.0, 60°C Archaeoglobus fulgidus
8
-
 indole wild-type, pH 8.0, 37°C Escherichia coli
19
-
 indole mutant M149T/N171D, pH 8.0, 37°C Escherichia coli
20
-
 indole mutant M144T/N166D, pH 8.0, 75°C Pyrococcus furiosus
20
-
 indole mutant P14L/P19G/I69V/L274S/T292S, pH 8.0, 75°C Thermotoga maritima
30
-
 indole mutant P12L/E17G/I68V/F274S/T292S/T321A, pH 8.0, 75°C Pyrococcus furiosus
31
-
 indole mutant M156T/N178D, pH 8.0, 60°C Archaeoglobus fulgidus
39
-
 indole wild-type, pH 8.0, 75°C Thermotoga maritima
100
-
 indole mutant M145T/N167D, pH 8.0, 75°C Thermotoga maritima
110
-
 indole mutant P25L/P30G/I80V/L285S/T303S/T321A, pH 8.0, 60°C Archaeoglobus fulgidus
230
-
 indole mutant T292S, pH 8.0, 75°C Thermotoga maritima
380
-
 indole mutant P19G/I69V/T292S, pH 8.0, 75°C Thermotoga maritima