Activating Compound | Comment | Organism | Structure |
---|---|---|---|
guanidine hydrochloride | stimulating, dual effector as cation activator and as a modulator of the active site conformation by alteration of the equilibrium distribution of pyridoxal 5'-phosphate intermediates formed in reaction with the beta-subunit, effects on reaction are highly dependent on the substrate, effects are altered by NaCl | Salmonella enterica subsp. enterica serovar Typhimurium |
Cloned (Comment) | Organism |
---|---|
expression of wild-type enzyme complex and of the beta-subunit dimer in Escherichia coli CB149 | Salmonella enterica subsp. enterica serovar Typhimurium |
General Stability | Organism |
---|---|
binding of allosteric ligands stabilizes the enzyme's conformation | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Urea | the beta-elimination activity of the enzyme complex decrease less sharply than the beta-replacement activity with increasing concentration in absence of NaCl, 0.1 M NaCl alters the effect of urea concentration | Salmonella enterica subsp. enterica serovar Typhimurium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | ligand binding sites, effector on the action of activators or inhibitors, overview | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type enzyme complex and of the beta-subunit dimer from Escherichia coli CB149 | Salmonella enterica subsp. enterica serovar Typhimurium |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O | also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8) | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(indol-3-yl)glycerol 3-phosphate | alpha-subunit of the bienzyme complex, alpha-reaction | Salmonella enterica subsp. enterica serovar Typhimurium | D-glyceraldehyde 3-phosphate + indole | - |
? | |
beta-chloro-L-alanine | in absence of indole | Salmonella enterica subsp. enterica serovar Typhimurium | pyruvate + NH3 + HCl | - |
? | |
beta-chloro-L-alanine + indole | - |
Salmonella enterica subsp. enterica serovar Typhimurium | L-tryptophan + HCl | - |
? | |
L-serine | - |
Salmonella enterica subsp. enterica serovar Typhimurium | pyruvate + NH3 | - |
? | |
L-serine + indole | beta-subunit of the bienzyme complex, beta-reaction | Salmonella enterica subsp. enterica serovar Typhimurium | L-tryptophan + H2O | - |
? | |
additional information | reaction intermediates formed in the different reactions, conformational transition states regulate the activity and specificity of the enzyme complex | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | conformational states, overview, subunit dissociation in presence of guanidinium hydrochloride | Salmonella enterica subsp. enterica serovar Typhimurium |
tetramer | alpha2beta2 complex | Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium |