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Literature summary for 4.2.1.154 extracted from
- Tetracenomycin F2 cyclase: intramolecular aldol condensation in the biosynthesis of tetracenomycin C in Streptomyces glaucescens (1993), Biochemistry, 32, 11149-11154.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.121 | - |
tetracenomycin F2 | at pH 8.0 and 30°C | Streptomyces glaucescens |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 12500 | - |
3 * 12500, SDS-PAGE | Streptomyces glaucescens |
| 12728 | - |
3 * 12728, calculated from amino acid sequence | Streptomyces glaucescens |
| 37500 | - |
gel filtration | Streptomyces glaucescens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tetracenomycin F2 | Streptomyces glaucescens | at pH 8.0 or higher, the enzyme catalyzes the cyclization of tetracenomycin F2 to tetracenomycin F1, while below pH 6.5, the enzyme catalyzes the cyclization of tetracenomycin F2 to 9-decarboxy-tetracenomycin F1 | tetracenomycin F1 + H2O | - |
? | |
| tetracenomycin F2 | Streptomyces glaucescens WMH1068 | at pH 8.0 or higher, the enzyme catalyzes the cyclization of tetracenomycin F2 to tetracenomycin F1, while below pH 6.5, the enzyme catalyzes the cyclization of tetracenomycin F2 to 9-decarboxy-tetracenomycin F1 | tetracenomycin F1 + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces glaucescens | - |
- |
- |
| Streptomyces glaucescens WMH1068 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, Mono Q column chromatography, phenyl-Superose gel filtration, Superose 6 gel filtration, and Sephacryl S-200 gel filtration | Streptomyces glaucescens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.000571 | - |
cell-free extract, at pH 8.0 and 30°C | Streptomyces glaucescens |
| 0.309 | - |
after 541fold purification, at pH 8.0 and 30°C | Streptomyces glaucescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tetracenomycin F2 | at pH 8.0 or higher, the enzyme catalyzes the cyclization of tetracenomycin F2 to tetracenomycin F1, while below pH 6.5, the enzyme catalyzes the cyclization of tetracenomycin F2 to 9-decarboxy-tetracenomycin F1 | Streptomyces glaucescens | tetracenomycin F1 + H2O | - |
? | |
| tetracenomycin F2 | at pH 8.0 or higher, the enzyme catalyzes the cyclization of tetracenomycin F2 to tetracenomycin F1, while below pH 6.5, the enzyme catalyzes the cyclization of tetracenomycin F2 to 9-decarboxy-tetracenomycin F1 | Streptomyces glaucescens WMH1068 | tetracenomycin F1 + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotrimer | 3 * 12500, SDS-PAGE | Streptomyces glaucescens |
| homotrimer | 3 * 12728, calculated from amino acid sequence | Streptomyces glaucescens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Tcm F2 cyclase | - |
Streptomyces glaucescens |
| tcmI | - |
Streptomyces glaucescens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8.5 | - |
Streptomyces glaucescens |
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