Cloned (Comment) | Organism |
---|---|
MurQ-His fusion protein is expressed in Escherichia coli BL21 cells | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HiTrap chelating HP column chromatography, gel filtration | Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, imidazole buffer (pH 8.0) by adding 20% glycerol and 1 mM dithiothreitol, at least several weeks, activity is retained | Escherichia coli |
4°C, purified etherase is only active for a few days | Escherichia coli |
temperature, storage medium, duration, loss of activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | N-acetylmuramate is not a substrate nor are anhydro-N-acetylmuramate or muramic acid | Escherichia coli | ? | - |
? | |
N-acetylmuramate 6-phosphate + H2O | - |
Escherichia coli | D-lactate + N-acetyl-D-glucosamine 6-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MurNAc etherase | - |
Escherichia coli |
MurQ | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
malfunction | a murQ deletion mutant cannot grow on N-acetylmuramic acid as the sole source of carbon | Escherichia coli |
physiological function | MurQ is required for growth on N-acetylmuramic acid | Escherichia coli |