BRENDA - Enzyme Database
show all sequences of 4.2.1.125

Heterologous biosynthesis of triterpenoid dammarenediol-II in engineered Escherichia coli

Li, D.; Zhang, Q.; Zhou, Z.; Zhao, F.; Lu, W.; Biotechnol. Lett. 38, 603-609 (2016)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene PNA, recombinant expression in Escherichia coli strain BL21(DE3), coexpression of squalene synthase (UniProt ID P29704), squalene epoxidase (UniProt ID P32476), and NADPH-cytochrome P450 reductase (UniProt ID P16603) from Saccharomyces cerevisiae, and squalene epoxidase (UniProt ID Q603D5) from Methylococcus capsulatus, as well as NADPH-cytochrome P450 reductase from Arabidopsis thaliana (UniProt ID Q9SB48)
Panax ginseng
Engineering
Protein Variants
Commentary
Organism
additional information
heterologous biosynthesis of triterpenoid dammarenediol-II, the precursor of dammarane-type tetracyclic ginsenosides, in engineered Escherichia coli strain BL21(DE3) by expressing the enzyme, and coexpressing squalene synthase, squalene epoxidase, and NADPH-cytochrome P450 reductase from Saccharomyces cerevisiae, and squalene epoxidase from Methylococcus capsulatus, as well as NADPH-cytochrome P450 reductase from Arabidopsis thaliana, and thereby reconstituting the 2,3-oxidosqualene-derived triterpenoid biosynthetic pathway. Method evaluation and optimization, overview
Panax ginseng
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(3S)-2,3-epoxy-2,3-dihydrosqualene + H2O
Panax ginseng
-
dammarenediol II
-
-
r
Organism
Organism
UniProt
Commentary
Textmining
Panax ginseng
Q08IT1
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(3S)-2,3-epoxy-2,3-dihydrosqualene + H2O
-
747390
Panax ginseng
dammarenediol II
-
-
-
r
Synonyms
Synonyms
Commentary
Organism
dammarenediol-II synthase
-
Panax ginseng
DDS
-
Panax ginseng
PNA
-
Panax ginseng
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
-
Panax ginseng
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Panax ginseng
Cloned(Commentary) (protein specific)
Commentary
Organism
gene PNA, recombinant expression in Escherichia coli strain BL21(DE3), coexpression of squalene synthase (UniProt ID P29704), squalene epoxidase (UniProt ID P32476), and NADPH-cytochrome P450 reductase (UniProt ID P16603) from Saccharomyces cerevisiae, and squalene epoxidase (UniProt ID Q603D5) from Methylococcus capsulatus, as well as NADPH-cytochrome P450 reductase from Arabidopsis thaliana (UniProt ID Q9SB48)
Panax ginseng
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
heterologous biosynthesis of triterpenoid dammarenediol-II, the precursor of dammarane-type tetracyclic ginsenosides, in engineered Escherichia coli strain BL21(DE3) by expressing the enzyme, and coexpressing squalene synthase, squalene epoxidase, and NADPH-cytochrome P450 reductase from Saccharomyces cerevisiae, and squalene epoxidase from Methylococcus capsulatus, as well as NADPH-cytochrome P450 reductase from Arabidopsis thaliana, and thereby reconstituting the 2,3-oxidosqualene-derived triterpenoid biosynthetic pathway. Method evaluation and optimization, overview
Panax ginseng
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(3S)-2,3-epoxy-2,3-dihydrosqualene + H2O
Panax ginseng
-
dammarenediol II
-
-
r
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(3S)-2,3-epoxy-2,3-dihydrosqualene + H2O
-
747390
Panax ginseng
dammarenediol II
-
-
-
r
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
-
Panax ginseng
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Panax ginseng
Other publictions for EC 4.2.1.125
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748118
Liu
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Molecular simulation and cata ...
Panax ginseng
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563-570
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747390
Li
Heterologous biosynthesis of ...
Panax ginseng
Biotechnol. Lett.
38
603-609
2016
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748874
Cheng
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SNPs of dammarenediol synthas ...
Panax ginseng
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17
194-200
2016
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Panax ginseng
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Wang
The isolation and characteriza ...
Panax quinquefolius
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Han
Production of dammarenediol-II ...
Panax ginseng
Plant Cell Rep.
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729358
Hu
Molecular cloning, expression, ...
Panax ginseng
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2013
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Heterologous expression of dam ...
Panax ginseng
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730586
Lee
Dammarenediol-II production co ...
Panax ginseng
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Panax ginseng
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