Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, recombinant expression of functional His-tagged enzyme in Escherichia coli strains Origami B (DE3) amd Rosetta (DE3) | Panax ginseng |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
84600 | - |
x * 84600, about, sequence calculation, x * 89000, recombinant His-tagged enzyme, SDS-PAGE | Panax ginseng |
89000 | - |
x * 84600, about, sequence calculation, x * 89000, recombinant His-tagged enzyme, SDS-PAGE | Panax ginseng |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3S)-2,3-epoxy-2,3-dihydrosqualene + H2O | Panax ginseng | - |
dammarenediol II | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Panax ginseng | Q08IT1 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strains Origami B (DE3) amd Rosetta (DE3) by nickel affinity chromatography | Panax ginseng |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Panax ginseng | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3S)-2,3-epoxy-2,3-dihydrosqualene + H2O | - |
Panax ginseng | dammarenediol II | - |
? | |
additional information | LC-MS analysis of substrate and product amounts | Panax ginseng | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 84600, about, sequence calculation, x * 89000, recombinant His-tagged enzyme, SDS-PAGE | Panax ginseng |
Synonyms | Comment | Organism |
---|---|---|
dammarenediol synthase | - |
Panax ginseng |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the ginsenoside biosynthetic pathway catalyzing the first committed step, the cyclization of 2,3-oxidosqualene to dammarenediol | Panax ginseng |
additional information | close correlation of dammarenediol synthetase and saponin production in vitro | Panax ginseng |