Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Li+ | liver enzyme is severely inhibited, muscle enzyme is moderately inhibited | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
2-phospho-D-glycerate | pH 6.8, muscle enolase | Sus scrofa | |
0.09 | - |
2-phospho-D-glycerate | pH 7.4, liver enolase and muscle enolase | Sus scrofa | |
0.17 | - |
2-phospho-D-glycerate | pH 6.8, liver enolase | Sus scrofa | |
0.17 | - |
phosphoenolpyruvate | pH 7.4, muscle enolase | Sus scrofa | |
0.19 | - |
phosphoenolpyruvate | pH 6.8, liver enolase | Sus scrofa | |
0.25 | - |
phosphoenolpyruvate | pH 7.4, liver enolase | Sus scrofa | |
0.33 | - |
phosphoenolpyruvate | pH 6.8, muscle enolase | Sus scrofa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | at pH 6.8, Km for liver enzyme: 0.67 mM, Km for muscle enzyme: 1.33 mM at pH 7.4, Km for liver enolase: 0.42 mM, Km for muscle enolase: 0.25 mM | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
2 * 44000, muscle enzyme, SDS-PAGE | Sus scrofa |
46000 | - |
2 * 46000, liver enzyme, SDS-PAGE | Sus scrofa |
84970 | - |
muscle enolase, calculation from amino acid composition | Sus scrofa |
85000 | - |
muscle enzyme, equilibrium sedimentation | Sus scrofa |
89020 | - |
liver enolase, calculation from amino acid composition | Sus scrofa |
92000 | - |
liver enzyme, equilibrium sedimentation | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
64.6 | - |
liver enolase | Sus scrofa |
91 | - |
muscle enolase | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | - |
Sus scrofa | phosphoenolpyruvate | - |
r | |
2-phospho-D-glycerate | - |
Sus scrofa | phosphoenolpyruvate + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 44000, muscle enzyme, SDS-PAGE | Sus scrofa |
dimer | 2 * 46000, liver enzyme, SDS-PAGE | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
muscle enolase, formation of 2-phosphoglycerate | Sus scrofa |
6.8 | - |
muscle enolase, formation of phosphoenolpyruvate | Sus scrofa |