Cloned (Comment) | Organism |
---|---|
gene cynS, cynS is presumably transcribed as part of the cynABDS operon, DNA and amino acid sequence determination and analysis, phylogenetic analysis | Prochlorococcus sp. |
gene cynS, DNA and amino acid sequence determination and analysis, phylogenetic analysis, overexpression in Escherichia coli strain HMS174 as maltose-binding-protein fusion protein | Synechococcus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Na-azide | - |
Prochlorococcus sp. | |
Na-azide | - |
Synechococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, recombinant MBP-CynS fusion enzyme, SDS-PAGE | Synechococcus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyanate + HCO3- + 2 H+ | Synechococcus sp. | - |
NH3 + 2 CO2 | - |
? | |
cyanate + HCO3- + 2 H+ | Prochlorococcus sp. | - |
NH3 + 2 CO2 | - |
? | |
cyanate + HCO3- + 2 H+ | Prochlorococcus sp. MED4 | - |
NH3 + 2 CO2 | - |
? | |
cyanate + HCO3- + 2 H+ | Synechococcus sp. WH7803 | - |
NH3 + 2 CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Prochlorococcus sp. | Q7V2U0 | gene cynS | - |
Prochlorococcus sp. MED4 | Q7V2U0 | gene cynS | - |
Synechococcus sp. | A5GPQ6 | gene cynS | - |
Synechococcus sp. WH7803 | A5GPQ6 | gene cynS | - |
Purification (Comment) | Organism |
---|---|
recombinant CynS-MBP from Escherichia coli strain HMS174 by amylose affinity chromatography | Synechococcus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.43 | - |
purified recombinant enzyme, pH 7.4, 55°C | Synechococcus sp. |
0.8 | - |
purified recombinant enzyme, pH 7.4, 4°C | Synechococcus sp. |
5.56 | - |
purified recombinant enzyme, pH 7.4, 26°C | Synechococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyanate + HCO3- + 2 H+ | - |
Synechococcus sp. | NH3 + 2 CO2 | - |
? | |
cyanate + HCO3- + 2 H+ | - |
Prochlorococcus sp. | NH3 + 2 CO2 | - |
? | |
cyanate + HCO3- + 2 H+ | - |
Prochlorococcus sp. MED4 | NH3 + 2 CO2 | - |
? | |
cyanate + HCO3- + 2 H+ | - |
Synechococcus sp. WH7803 | NH3 + 2 CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, recombinant MBP-CynS fusion enzyme, SDS-PAGE | Synechococcus sp. |
Synonyms | Comment | Organism |
---|---|---|
cyanate lyase | - |
Synechococcus sp. |
CynS | - |
Prochlorococcus sp. |
CynS | - |
Synechococcus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
26 | - |
- |
Synechococcus sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 55 | activity range | Synechococcus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 55 | CynS is rapidly inactivated at higher temperatures and only residual activity o f 0.09 U/mg occurs at 50°C | Synechococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Synechococcus sp. |
General Information | Comment | Organism |
---|---|---|
additional information | transcriptional regulation of cynS expression, overview | Synechococcus sp. |
additional information | transcriptional regulation of cynS expression, overview | Prochlorococcus sp. |