Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bicarbonate | - |
Synechococcus sp. | |
bicarbonate | - |
Synechocystis sp. | |
NtcA | transcription factor, global nitrogen regulator of cyanobacteria, required for expression | Synechococcus sp. | |
NtcA | transcription factor, global nitrogen regulator of cyanobacteria, required for expression | Synechocystis sp. |
Cloned (Comment) | Organism |
---|---|
gene cynS, DNA and amino acid sequence determination and analysis, tightly clustered with 2 genes located upstream, which encode proteins similar to the subunits of nitrate-nitrite transporter, complementation of deficient Escherichia coli strain | Synechococcus sp. |
gene cynS, tightly clustered with 4 putative molybdenum cofactor biosynthesis genes located downstream | Synechocystis sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NH4+ | negative regulation, represses enzyme expression | Synechococcus sp. | |
NH4+ | negative regulation, represses enzyme expression | Synechocystis sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16362 | - |
x * 16362, amino acid sequence calculation | Synechococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechococcus sp. | - |
strain PCC 7942, gene cynS | - |
Synechocystis sp. | - |
strain PCC 6803, gene cynS | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
cyanate + HCO3- + 2 H+ = NH3 + 2 CO2 | the enzyme requires bicarbonate as a cofactor. Its mechanism is to catalyse the attack of bicarbonate on cyanate, with elimination of carbon dioxide, thus catalysing hydration of the cyanate to carbamae. The carbamate spontaneously hydrolyses to ammonia and carbon dioxide | Synechococcus sp. | |
cyanate + HCO3- + 2 H+ = NH3 + 2 CO2 | the enzyme requires bicarbonate as a cofactor. Its mechanism is to catalyse the attack of bicarbonate on cyanate, with elimination of carbon dioxide, thus catalysing hydration of the cyanate to carbamate. The carbamate spontaneously hydrolyses to ammonia and carbon dioxide | Synechocystis sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.047 | - |
complemented deficient Escherichia coli cells | Synechococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NCO- + HCO3- | - |
Synechococcus sp. | NH4+ + CO2 | - |
? | |
NCO- + HCO3- | - |
Synechocystis sp. | NH4+ + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 16362, amino acid sequence calculation | Synechococcus sp. |
Synonyms | Comment | Organism |
---|---|---|
cyanase | - |
Synechococcus sp. |
cyanase | - |
Synechocystis sp. |
EC 4.3.99.1 | formerly | Synechococcus sp. |
EC 4.3.99.1 | formerly | Synechocystis sp. |