Literature summary for 4.1.99.13 extracted from

Guzman-Moreno, J.; Flores-Martinez, A.; Brieba, L.G.; Herrera-Estrella, A.
The Trichoderma reesei Cry1 protein is a member of the cryptochrome/photolyase family with 6-4 photoproduct repair activity (2014), PLoS ONE, 9, e100625 .

Search for more literature for 4.1.99.13

Cloned(Commentary)

Cloned (Comment)	Organism
gene cry1, sequence comparisons and phylogenetic analysis	Trichoderma reesei

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of a cry1 gene replacement mutants and cry1 overexpressing strains, (DELTAcry1, OEcry1, DELTAblr1 and DELTAenv1 strains). The mutant strains and the parental strain QM9414 are grown under continuous exposure to blue light or in the dark for 72 h at 28°C. The results show that neither the gene replacement mutant nor the overexpressing strain have any colony morphology alterations, and conidiated normally, the two independent gene replacement mutants and overexpressing strains show the same unaltered behavior compared to wild-type	Trichoderma reesei

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(6-4) photoproduct (in DNA)	Trichoderma reesei	-	2 pyrimidine residues (in DNA)	-	?
(6-4) photoproduct (in DNA)	Trichoderma reesei QM6a	-	2 pyrimidine residues (in DNA)	-	?

Organism

Organism	UniProt	Comment	Textmining
Trichoderma reesei	G0RI60	i.e. Hypocrea jecorina strain QM6a	-
Trichoderma reesei QM6a	G0RI60	i.e. Hypocrea jecorina strain QM6a	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
conidium	-	Trichoderma reesei	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(6-4) photoproduct (in DNA)	-	Trichoderma reesei	2 pyrimidine residues (in DNA)	-	?
(6-4) photoproduct (in DNA)	-	Trichoderma reesei QM6a	2 pyrimidine residues (in DNA)	-	?
additional information	Cry1, heterologously expressed and purified from Escherichia coli, is capable of binding to undamaged and 6-4PP damaged DNA. Cry1 repairs 6-4PP, but not CPD and Dewar DNA lesions	Trichoderma reesei	?	-	?
additional information	Cry1, heterologously expressed and purified from Escherichia coli, is capable of binding to undamaged and 6-4PP damaged DNA. Cry1 repairs 6-4PP, but not CPD and Dewar DNA lesions	Trichoderma reesei QM6a	?	-	?

Subunits

Subunits	Comment	Organism
More	the N-terminal region of Cry1 contains a conserved DNA photolyase domain (Pfam00875) between amino acid positions 6 and 173, and a FAD-binding domain (Pfam03441) in the amino acid positions 239 to 549, involved in binding a light harvesting cofactor, while the C-terminal domain (residues 550 to 628) does not show similarity to any known domain, homology structure modeling, structure comparisons, overview	Trichoderma reesei

Synonyms

Synonyms	Comment	Organism
Cry1	-	Trichoderma reesei
TRIREDRAFT_77473	locus name	Trichoderma reesei

Expression

Organism	Comment	Expression
Trichoderma reesei	cry1 mRNA accumulates in response to blue-light in a blr1-dependent manner and is modulated by Envoy	up

General Information

General Information	Comment	Organism
evolution	the Trichoderma reesei Cry1 protein is a member of the cryptochrome/photolyase family with 6-4 photoproduct repair activity. The two major types of DNA damage are cyclobutane pyrimidine dimers (CPD) and 6-4 photoproducts (6-4PP), which are repaired under illumination by CPD and 6-4 photolyases, respectively. Phylogenetic analysis and tree, overview	Trichoderma reesei
malfunction	conidia of cry1 mutants show decreased photorepair capacity of DNA damage caused by UV light. In contrast, strains overexpressing Cry1 show increased repair, as compared to the parental strain even in the dark	Trichoderma reesei
additional information	homology modeling using the crystal structure of a photolyase from Drosophila melanogaster in complex with 6--4 thymine dimer, PDB ID 3CVU, as a template	Trichoderma reesei
physiological function	Cry1 protein is a member of the cryptochrome/photolyase family with 6-4 photoproduct repair activity, it uses UV-visible light to repair DNA damage caused by UV radiation. The type of DNA damages, which are repaired under illumination by 6-4 photolyase, are 6-4 photoproducts (6-4PP). Cry1 is involved in the process of photoreactivation in Trichoderma reesei but has no participation in aspects related to growth at least under the tested conditions	Trichoderma reesei

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Release 2023.1 (January 2023)