Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coli strains XL-1 Blue and M15 | Methanocaldococcus jannaschii |
Protein Variants | Comment | Organism |
---|---|---|
D21E | site-directed mutagenesis, inactive mutant | Methanocaldococcus jannaschii |
D21N | site-directed mutagenesis, inactive mutant | Methanocaldococcus jannaschii |
D30E | site-directed mutagenesis, inactive mutant | Methanocaldococcus jannaschii |
E185D | site-directed mutagenesis, inactive mutant | Methanocaldococcus jannaschii |
E26D | site-directed mutagenesis, inactive mutant | Methanocaldococcus jannaschii |
E26Q | site-directed mutagenesis, inactive mutant | Methanocaldococcus jannaschii |
E28D | site-directed mutagenesis, inactive mutant | Methanocaldococcus jannaschii |
H164N | site-directed mutagenesis, inactive mutant | Methanocaldococcus jannaschii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25799 | - |
2 * 25799, recombinant enzyme, mass spectrometry and SDS-PAGE | Methanocaldococcus jannaschii |
51600 | - |
recombinant enzyme, analytical ultrafiltration | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 5-phosphate | Methanocaldococcus jannaschii | - |
formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | - |
orf MJ0055 | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli by hydrophobic interaction and hydroxyapatite chromatography, and ultrafiltration | Methanocaldococcus jannaschii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate | reaction mechanism involving intramolecular skeletal rearrangement, a cluster of charged amino acid residues comprising Arg25, Glu26 and Glu28, Asp21 and Asp30 is essential for catalytic activity, as well as His164 and Glu185 | Methanocaldococcus jannaschii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.174 | - |
purified enzyme | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 5-phosphate | - |
Methanocaldococcus jannaschii | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? | |
D-ribulose 5-phosphate | the reaction involves an intramolecular skeletal rearrangement, NMR study | Methanocaldococcus jannaschii | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 25799, recombinant enzyme, mass spectrometry and SDS-PAGE | Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 7.7 | assay at | Methanocaldococcus jannaschii |