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Literature summary for 4.1.3.27 extracted from

  • Hertel, S.C.; Hieke, M.; Gröger, D.
    Anthranilate synthase from Ruta graveolens: partial purification and properties (1991), Biochem. Physiol. Pflanz., 187, 121-129.
No PubMed abstract available
Search for more literature for 4.1.3.27

Inhibitors

Inhibitors Comment Organism Structure
Ba2+
-
 Ruta graveolens
Co2+ 10 mM, 67% inhibition of enzyme from cell line R-15, 33% inhibition of enzyme from cell line R-20 Ruta graveolens
Cu2+
-
 Ruta graveolens
Hg2+ 1 mM HgCl2, 94.1% inhibition Ruta graveolens
L-Trp competitive with respect to chorismate Ruta graveolens
MnCl2
-
 Ruta graveolens
Pb2+
-
 Ruta graveolens
PCMB 1 mM, 88.6% inhibition Ruta graveolens
Zn2+
-
 Ruta graveolens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
 Gln cell line R-15 Ruta graveolens
0.22
-
 chorismate cell line R-20 Ruta graveolens
0.25
-
 Gln cell line R-20 Ruta graveolens
0.28
-
 chorismate cell line R-15 Ruta graveolens

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ at 1-5 mM Ruta graveolens
Fe2+ 1 mM, can partially replace Mg2+ in activation Ruta graveolens
Mg2+
-
 Ruta graveolens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
220000
-
 gel filtration Ruta graveolens

Organism

Organism UniProt Comment Textmining
Ruta graveolens
-
 a high rutacridone producing cell line R-20 and low rutacrinone producing cell line R-15
-

Purification (Commentary)

Purification (Comment) Organism
partial, from a high rutacridone producing cell line R-20 and from a low rutacrinone producing cell line R-15 Ruta graveolens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Ruta graveolens

Storage Stability

Storage Stability Organism
-20°C, unstable Ruta graveolens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chorismate + L-Gln
-
 Ruta graveolens anthranilate + pyruvate + L-glutamate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37 40
-
 Ruta graveolens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 45 25°C: about 70% of maximal activity, 45°C: about 85% of maximal activity Ruta graveolens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 5 min, about 25% loss of activity Ruta graveolens
40
-
 5 min, about 50% loss of activity Ruta graveolens
45
-
 5 min, about 80% loss of activity Ruta graveolens
50
-
 5 min, about 90% loss of activity Ruta graveolens