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Literature summary for 4.1.3.27 extracted from
- Anthranilate synthetase from Serratia marcescens (1971), J. Biol. Chem., 246, 6899-6907.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 6-diazo-5-oxo-L-norleucine | inhibition of Gln-dependent activity, no inactivation of NH4+-dependent activity | Serratia marcescens |  |
| iodoacetamide | inhibition of Gln-dependent activity, no inactivation of NH4+-dependent activity | Serratia marcescens | |
| L-Trp | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| L-Trp | - |
Serratia marcescens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
chorismate | with NH4+ as cosubstrate | Serratia marcescens | |
| 0.018 | - |
chorismate | with Gln as cosubstrate | Serratia marcescens | |
| 0.5 | - |
Gln | - |
Serratia marcescens | |
| 5.9 | - |
NH4+ | - |
Serratia marcescens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 21000 | - |
ASI, ASII, 2 * 21000 + 2 * 60000, SDS-PAGE | Serratia marcescens |
| 60000 | - |
ASI, ASII, 2 * 21000 + 2 * 60000, SDS-PAGE | Serratia marcescens |
| 141000 | - |
sucrose density gradient centrifugation | Serratia marcescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
multifunctional anthranilate synthetase-anthranilate 5-phosphoribosylpyrophosphate phosphoribosyltransferase | - |
| Serratia marcescens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Serratia marcescens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Serratia marcescens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 2°C or -10°C, activity declines to 3400 or 4000 units per mg and remains constant for several months | Serratia marcescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chorismate + L-Gln | - |
Salmonella enterica subsp. enterica serovar Typhimurium | anthranilate + pyruvate + L-glutamate | - |
? | |
| chorismate + L-Gln | - |
Serratia marcescens | anthranilate + pyruvate + L-glutamate | - |
? | |
| chorismate + NH4+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| chorismate + NH4+ | - |
Serratia marcescens | ? | - |
? | |
| additional information | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| additional information | glutaminase activity | Serratia marcescens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | multifunctional anthranilate synthetase-anthranilate 5-phosphoribosylpyrophosphate phosphoribosyltransferase | Salmonella enterica subsp. enterica serovar Typhimurium |
| tetramer | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
| tetramer | ASI, ASII, 2 * 21000 + 2 * 60000, SDS-PAGE | Serratia marcescens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
- |
Serratia marcescens |
| 8.5 | - |
- |
Serratia marcescens |
| 8.5 | - |
NH4+-dependent activity | Salmonella enterica subsp. enterica serovar Typhimurium |
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