Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Chloroflexus aurantiacus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | inhibits at 1 mM | Chloroflexus aurantiacus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.061 | - |
(3S)-3-carboxy-3-hydroxypropanoyl-CoA | pH 7.1, 55°C | Chloroflexus aurantiacus | |
0.089 | - |
beta-methylmalyl-CoA | pH 7.1, 55°C | Chloroflexus aurantiacus | |
0.36 | - |
acetyl-CoA | pH 7.1, 55°C | Chloroflexus aurantiacus | |
1.2 | - |
propionyl-CoA | pH 7.1, 55°C | Chloroflexus aurantiacus | |
2 | - |
glyoxylate | pH 7.1, 55°C | Chloroflexus aurantiacus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 4 mM, requirement | Chloroflexus aurantiacus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
x * 38000, alpha5 or alpha6, SDS-PAGE | Chloroflexus aurantiacus |
210000 | - |
gel filtration | Chloroflexus aurantiacus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3S)-3-carboxy-3-hydroxypropanoyl-CoA | Chloroflexus aurantiacus | - |
acetyl-CoA + glyoxylate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chloroflexus aurantiacus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Chloroflexus aurantiacus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.48 | - |
acetyl-CoA condensation | Chloroflexus aurantiacus |
0.5 | - |
propionyl-CoA condensation | Chloroflexus aurantiacus |
2.3 | - |
L-malyl-CoA lyase activity | Chloroflexus aurantiacus |
6.5 | - |
beta-methylmalyl-CoA lyase activity | Chloroflexus aurantiacus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3S)-3-carboxy-3-hydroxypropanoyl-CoA | - |
Chloroflexus aurantiacus | acetyl-CoA + glyoxylate | - |
r | |
(3S)-3-carboxy-3-hydroxypropanoyl-CoA | malyl-CoA | Chloroflexus aurantiacus | acetyl-CoA + glyoxylate | - |
r | |
propionyl-CoA + glyoxylate | - |
Chloroflexus aurantiacus | erythro-beta-methylmalyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000, alpha5 or alpha6, SDS-PAGE | Chloroflexus aurantiacus |
Synonyms | Comment | Organism |
---|---|---|
L-malyl-CoA lyase/beta-methylmalyl-CoA lyase | the two enzymes are identical and both enzymatic reactions are catalysed by one single bifunctional enzyme | Chloroflexus aurantiacus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Chloroflexus aurantiacus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
stable for 20 min | Chloroflexus aurantiacus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.8 | - |
(3S)-3-carboxy-3-hydroxypropanoyl-CoA | pH 7.1, 55°C | Chloroflexus aurantiacus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
at 55°C | Chloroflexus aurantiacus |