Cloned (Comment) | Organism |
---|---|
DNA fragment carrying the gene proA from chromosomal DNA cloned and overproduced in Escherichia coli XL1-Blue MRF' | Pseudomonas straminea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
L-4-carboxy-4-hydroxy-2-oxoadipate | pH 8.0, 25°C | Pseudomonas straminea | |
0.044 | - |
DL-4-carboxy-4-hydroxy-2-oxoadipate | pH 8.0, 25°C | Pseudomonas straminea | |
0.15 | - |
L-4-carboxy-4-hydroxy-2-oxoadipate | pH 8.0, 25°C | Pseudomonas straminea | |
0.24 | - |
DL-4-hydroxy-2-oxoglutarate | pH 8.0, 25°C | Pseudomonas straminea | |
0.5 | - |
oxaloacetate | pH 8.0, 25°C | Pseudomonas straminea | |
1.25 | - |
DL-4-hydroxy-4-methyl-2-oxoglutarate | pH 8.0, 25°C | Pseudomonas straminea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can replace Mg2+ | Pseudomonas straminea | |
Mg2+ | virtually no activity in absence of Mg2+ or Pi | Pseudomonas straminea | |
Mn2+ | can replace Mg2+ | Pseudomonas straminea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24070 | - |
calculated from amino acid sequence corresponding to gene sequence | Pseudomonas straminea |
26000 | - |
6 * 26000, homohexamer, SDS-PAGE | Pseudomonas straminea |
160000 | - |
gel filtration | Pseudomonas straminea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxy-4-methyl-2-oxoglutarate | Pseudomonas straminea | involved in biosynthesis of gamma-substituted glutamic acid, participates in the alpha-keto acid pathway for degradation of the meta-fission product of protocatechuate, in Pseudomonas ochraceae grown on phthalate the physiolological substrate is not hydroxymethyl glutamic acid but CHA, L-4-carboxy-4-hydroxy-2-oxoadipate, which is cleaved to pyruvate and oxaloacetate | pyruvate | - |
r | |
l-4-carboxy-4-hydroxy-2-oxoadipate | Pseudomonas straminea | - |
pyruvate + oxaloacetate | - |
? | |
l-4-carboxy-4-hydroxy-2-oxoadipate | Pseudomonas straminea NGJ1 | - |
pyruvate + oxaloacetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas straminea | - |
- |
- |
Pseudomonas straminea NGJ1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and recombinant enzyme | Pseudomonas straminea |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
870 | - |
- |
Pseudomonas straminea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxy-4-methyl-2-oxoglutarate | involved in biosynthesis of gamma-substituted glutamic acid, participates in the alpha-keto acid pathway for degradation of the meta-fission product of protocatechuate, in Pseudomonas ochraceae grown on phthalate the physiolological substrate is not hydroxymethyl glutamic acid but CHA, L-4-carboxy-4-hydroxy-2-oxoadipate, which is cleaved to pyruvate and oxaloacetate | Pseudomonas straminea | pyruvate | - |
r | |
D-4-carboxy-4-hydroxy-2-oxoadipate | - |
Pseudomonas straminea | pyruvate + oxaloacetate | - |
? | |
D-4-carboxy-4-hydroxy-2-oxoadipate | - |
Pseudomonas straminea NGJ1 | pyruvate + oxaloacetate | - |
? | |
DL-4-carboxy-4-hydroxy-2-oxoadipate | - |
Pseudomonas straminea | pyruvate + oxaloacetate | - |
? | |
DL-4-carboxy-4-hydroxy-2-oxoadipate | - |
Pseudomonas straminea NGJ1 | pyruvate + oxaloacetate | - |
? | |
DL-4-hydroxy-2-oxoglutarate | - |
Pseudomonas straminea | pyruvate + glyoxylate | - |
? | |
DL-4-hydroxy-4-methyl-2-oxoglutarate | - |
Pseudomonas straminea | pyruvate | - |
? | |
DL-4-hydroxy-4-methyl-2-oxoglutarate | - |
Pseudomonas straminea NGJ1 | pyruvate | - |
? | |
l-4-carboxy-4-hydroxy-2-oxoadipate | - |
Pseudomonas straminea | pyruvate + oxaloacetate | - |
? | |
l-4-carboxy-4-hydroxy-2-oxoadipate | - |
Pseudomonas straminea NGJ1 | pyruvate + oxaloacetate | - |
? | |
oxaloacetate | decarboxylation | Pseudomonas straminea | ? | - |
? | |
oxaloacetate | decarboxylation | Pseudomonas straminea NGJ1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 26000, homohexamer, SDS-PAGE | Pseudomonas straminea |
Synonyms | Comment | Organism |
---|---|---|
4-hydroxy-4-methyl-2-oxoglutarate aldolase | - |
Pseudomonas straminea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudomonas straminea |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas straminea | isoelectric focusing | - |
5 |
Pseudomonas straminea | predicted pI | - |
5.7 |