Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Gloeothece citriformis PCC 7424 |
General Stability | Organism |
---|---|
freezing and thawing reduce the enzyme activity significantly | Gloeothece citriformis PCC 7424 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphoenolpyruvate | 90% inhibition at 2 mM | Gloeothece citriformis PCC 7424 | |
succinate | 90% inhibition at 5 mM | Gloeothece citriformis PCC 7424 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
DL-isocitrate | at pH 7.4 and 25°C | Gloeothece citriformis PCC 7424 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, in the absence of Mg2+, no enzymatic activity is measured | Gloeothece citriformis PCC 7424 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate | Gloeothece citriformis PCC 7424 | - |
succinate + glyoxylate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gloeothece citriformis PCC 7424 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
glutathione agarose column chromatography | Gloeothece citriformis PCC 7424 |
Storage Stability | Organism |
---|---|
4°C, 50 mM Tris/HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 1 mM dithiothreitol, few days, the enzyme remains stable | Gloeothece citriformis PCC 7424 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-isocitrate | - |
Gloeothece citriformis PCC 7424 | succinate + glyoxylate | - |
? | |
isocitrate | - |
Gloeothece citriformis PCC 7424 | succinate + glyoxylate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 54000, SDS-PAGE | Gloeothece citriformis PCC 7424 |
? | x * 54500, calculated from amino acid sequence | Gloeothece citriformis PCC 7424 |
Synonyms | Comment | Organism |
---|---|---|
ICL | - |
Gloeothece citriformis PCC 7424 |
Organism | Comment | Expression |
---|---|---|
Gloeothece citriformis PCC 7424 | enzyme activity is induced under alternating day/night cycles | up |