Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.3.1 extracted from

  • Eprintsev, A.; Dyachenko, E.; Lykova, T.; Kuen, C.; Popov, V.
    Preparation and properties of isocitrate lyase isoforms from the cotyledons of Glycine max L (2010), Appl. Biochem. Microbiol., 46, 93-98.
    View publication on PubMed
Search for more literature for 4.1.3.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 isocitrate pH 7.5, 25°C Glycine max
0.05
-
 isocitrate pH 7.5, 25°C Glycine max

Localization

Localization Comment Organism GeneOntology No. Textmining
glyoxysome
-
 Glycine max 9514
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
 4 * 66000, SDS-PAGE, gel filtration Glycine max
246000
-
 gel filtration Glycine max
272000
-
 gel filtration Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate frationation, gel filtration, ion exchange chromatography (DEAE-cellulose) Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
 Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.28
-
 pH 7.5, 25°C Glycine max
5.81
-
 pH 7.5, 25°C Glycine max

Storage Stability

Storage Stability Organism
-20°C, Tris-HCl-buffer, pH 7.5, 50% loss of activity after 3 days, 100% loss of activity after 6 days Glycine max
-70°C, Tris-HCl-buffer, pH 7.5, 5% loss of activity after 1 day, 10% loss of activity for each following day Glycine max
-70°C, Tris-HCl-buffer, pH 7.5, 80% activity by the tenth day of storage in the presence of 5 mM Mg2+ and 25% of activity when 5 mM Mn2+ are used for stabilization, the following storage conditions are optimal: low temperature (-70°C), 25% glycerol in the medium and the presence of 5 mM MgCl2 Glycine max
-70°C, Tris-HCl-buffer, pH 7.5, 85% activity by the tenth day of storage in the presence of 5 mM Mn2+ and 30% of activity when 5 mM Mg2+ are used for stabilization, the following storage conditions are optimal: low temperature (-70°C), 25% glycerol in the medium and the presence of 5 mM MnCl2 Glycine max
room temperature, Tris-HCl-buffer, pH 7.5, 80% loss of activity in 24 hours Glycine max
storage in glycerol-containing medium (25% glycerol) results in a 30% higher activity than storage in the absence of glycerol Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate
-
 Glycine max glyoxylate + succinate
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 66000, SDS-PAGE, gel filtration Glycine max

Synonyms

Synonyms Comment Organism
isocitrate lyase 1 relative electrophoretic mobility Rf 0.28 Glycine max
isocitrate lyase 2 relative electrophoretic mobility Rf 0.44 Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Glycine max
8
-
-
 Glycine max