KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.056 | - |
isocitrate | ICL1 | Zea mays | |
0.083 | - |
isocitrate | ICL2 | Zea mays | |
0.1 | - |
isocitrate | ICL | Zea mays |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | ICL1, dependent on | Zea mays | |
Mn2+ | ICL2 and ICL, dependent on | Zea mays |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
164000 | - |
gel filtration, ICL1 | Zea mays |
207000 | - |
gel filtration, ICL2 | Zea mays |
208000 | - |
gel filtration, ICL | Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, purification of ICL1 is 123fold with a yield of 5.7%, purification degree and yield of ICL2 are 110 and 5.7%, respectively | Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | ICL | Zea mays | - |
scutellum | ICL1 and and ICL2 | Zea mays | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.04 | - |
homogenate | Zea mays |
4.4 | - |
ICL2, 1003fold purified | Zea mays |
4.9 | - |
ICL1, 123fold purified | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate | ICL1, ICL2 and ICL | Zea mays | succinate + glyoxylate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | gel filtration, ICL1, ICL2 and ICL | Zea mays |
Synonyms | Comment | Organism |
---|---|---|
ICL | - |
Zea mays |
isocitrate lyase | - |
Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
ICL2 | Zea mays |
7.5 | - |
ICL1 | Zea mays |
Organism | Comment | Expression |
---|---|---|
Zea mays | Ca2+, K+, and Na+ have little effect on the regulation of activity of ICL isoforms | additional information |
General Information | Comment | Organism |
---|---|---|
metabolism | participation of isoforms in metabolic regulation of the glyoxylate cycle, organic acid metabolism during photorespiration in leaves and acidosis in corn seeds | Zea mays |