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Literature summary for 4.1.2.48 extracted from
- The GLY1 gene of Saccharomyces cerevisiae encodes a low-specific L-threonine aldolase that catalyzes cleavage of L-allo-threonine and L-threonine to glycine (1997), Eur. J. Biochem., 245, 289-293.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloned into pUCl18, and expressed in Escherichia coli | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10 | - |
L-allo-threonine | pH 8.0, 30°C | Saccharomyces cerevisiae |  |
| 55 | - |
L-threonine | pH 8.0, 30°C | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
4 * 41000, SDS-PAGE | Saccharomyces cerevisiae |
| 42000 | - |
4 * 42000, calculated from sequence | Saccharomyces cerevisiae |
| 170000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | P37303 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.5 | - |
pH 8.0, 30°C | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-allo-threonine | - |
Saccharomyces cerevisiae | glycine + acetaldehyde | - |
? | |
| L-threonine | - |
Saccharomyces cerevisiae | glycine + acetaldehyde | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 41000, SDS-PAGE | Saccharomyces cerevisiae |
| homotetramer | 4 * 42000, calculated from sequence | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GLY1 | - |
Saccharomyces cerevisiae |
| L-TA | - |
Saccharomyces cerevisiae |
| low specificity L-TA | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Saccharomyces cerevisiae |
| 55 | - |
aldehyde formation from L-allo-threonine | Saccharomyces cerevisiae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
15 min, enzyme retains only 25% of activity | Saccharomyces cerevisiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 41 | - |
L-allo-threonine | pH 8.0, 30°C | Saccharomyces cerevisiae | |
| 43 | - |
L-threonine | pH 8.0, 30°C | Saccharomyces cerevisiae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Saccharomyces cerevisiae |
| 8.5 | 9 | aldehyde formation from L-allo-threonine | Saccharomyces cerevisiae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 10 | 30 min, stable | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | 2 mol pyridoxal 5'-phosphate per 4 mol of subunit | Saccharomyces cerevisiae | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.78 | - |
L-threonine | pH 8.0, 30°C | Saccharomyces cerevisiae | |
| 4.1 | - |
L-allo-threonine | pH 8.0, 30°C | Saccharomyces cerevisiae | |
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