Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.2.47 extracted from

  • Narayanan, N.N.; Ihemere, U.; Ellery, C.; Sayre, R.T.
    Overexpression of hydroxynitrile lyase in cassava roots elevates protein and free amino acids while reducing residual cyanogen levels (2011), PLoS ONE, 6, e21996.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.2.47

Application

Application Comment Organism
food industry root-specific expression of cassava HNL not only increases total root protein levels 3fold approaching the target values for a nutritionally balanced meal but accelerates cyanogenesis during food processing resulting in a safer and more nutritious food product Manihot esculenta

Cloned(Commentary)

Cloned (Comment) Organism
expressed in roots of Manihot esculenta cultivar TMS-60444 Manihot esculenta

Localization

Localization Comment Organism GeneOntology No. Textmining
apoplast
-
 Manihot esculenta 48046
-

Organism

Organism UniProt Comment Textmining
Manihot esculenta
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Manihot esculenta
-
root
-
 Manihot esculenta
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-mandelonitrile
-
 Manihot esculenta cyanide + benzaldehyde
-
 ?
2-hydroxy-2-methylpropanenitrile
-
 Manihot esculenta cyanide + acetone
-
 ?

Synonyms

Synonyms Comment Organism
HNL
-
 Manihot esculenta
Hydroxynitrile lyase
-
 Manihot esculenta

Expression

Organism Comment Expression
Manihot esculenta the overexpression of HNL driven by the patatin (root-specific) promoter results in a 2-20fold increase in relative mRNA expression in roots when compared with wild type, and a 5-6fold increase in expression when compared with CaMV 35S HNL transgenic lines up