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Literature summary for 4.1.2.47 extracted from

  • Rustler, S.; Motejadded, H.; Altenbuchner, J.; Stolz, A.
    Simultaneous expression of an arylacetonitrilase from Pseudomonas fluorescens and a (S)-oxynitrilase from Manihot esculenta in Pichia pastoris for the synthesis of (S)-mandelic acid (2008), Appl. Microbiol. Biotechnol., 80, 87-97.
    View publication on PubMed
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Application

Application Comment Organism
synthesis recombinant Pichia pastoris strains are constructed which simultaneously express the (S)-oxynitrilase of Manihot esculenta and the arylacetonitrilase of Pseudomonas fluorescens EBC191 each under the control of individual AOX1 promoters in order to obtain a whole cell catalyst for the synthesis of (S)-mandelic acid from benzaldehyde and cyanideproduction of optically active cyanohydrin compounds Manihot esculenta

Cloned(Commentary)

Cloned (Comment) Organism
recombinant Pichia pastoris strains are constructed which simultaneously expressed the (S)-oxynitrilase of Manihot esculenta and the arylacetonitrilase of Pseudomonas fluorescens EBC191 each under the control of individual AOX1 promoters in order to obtain a whole cell catalyst for the synthesis of (S)-mandelic acid from benzaldehyde and cyanide production of optically active cyanohydrin compounds Manihot esculenta

Organism

Organism UniProt Comment Textmining
Manihot esculenta
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyanide + benzaldehyde
-
 Manihot esculenta (S)-mandelonitrile
-
 ?

Synonyms

Synonyms Comment Organism
(S)-Oxynitrilase
-
 Manihot esculenta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Manihot esculenta

pH Range

pH Minimum pH Maximum Comment Organism
3 7
-
 Manihot esculenta