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Literature summary for 4.1.2.47 extracted from

  • Yan, G.; Cheng, S.; Zhao, G.; Wu, S.; Liu, Y.; Sun, W.
    A single residual replacement improves the folding and stability of recombinant cassava hydroxynitrile lyase in E. coli (2003), Biotechnol. Lett., 25, 1041-1047.
    View publication on PubMed
Search for more literature for 4.1.2.47

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) Manihot esculenta

Protein Variants

Protein Variants Comment Organism
G113S enhanced thermal stability compared to wild-type enzyme, mutant enzyme retains slight higher activity than the wild-type enzyme in an acidic environment, so the mutant enzyme maybe more effective for synthesis of (S)-cyanohydrin than the wild-type enzyme Manihot esculenta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
 mandelonitrile pH 5.4, 25°C, mutant enzyme G113S Manihot esculenta
1.4
-
 mandelonitrile pH 5.4, 25°C, wild-type enzyme Manihot esculenta

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 4 * 29000, SDS-PAGE Manihot esculenta

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
mandelonitrile Manihot esculenta
-
 HCN + benzaldehyde
-
 ?

Organism

Organism UniProt Comment Textmining
Manihot esculenta
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Manihot esculenta

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
35.6
-
 wild-type enzyme Manihot esculenta
57.2
-
 mutant enzyme G113S Manihot esculenta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
mandelonitrile
-
 Manihot esculenta HCN + benzaldehyde
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 29000, SDS-PAGE Manihot esculenta

Synonyms

Synonyms Comment Organism
(S)-HNL
-
 Manihot esculenta
HNL
-
 Manihot esculenta
HNL1
-
 Manihot esculenta
Hydroxynitrile lyase
-
 Manihot esculenta
MeHNL
-
 Manihot esculenta
mut-HNL1
-
 Manihot esculenta

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Manihot esculenta

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 70
-
 Manihot esculenta

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 mutant enzyme and wild-type enzyme G113S are stable for 20 min at pH 5.4 Manihot esculenta
60
-
 20 min at pH 5.4, mutant enzyme G113S loses about 10% of its initial activity, wild-type enzyme loses about 20% of its initial activity. More than 50% loss of activity of wild-type enzyme after 50 min, 10% loss of activity of mutant enzyme G113 after 50 min, 75% loss of activity of wild-type enzyme after 120 min, 25% loss of activity of mutant enzyme G113 after 120 min Manihot esculenta
65
-
 20 min at pH 5.4, mutant enzyme G113S loses about 15% of its initial activity, wild-type enzyme loses about 50% of its initial activity Manihot esculenta
70
-
 20 min at pH 5.4, about 80% loss of activity, mutant enzyme G113S and wild-type enzyme Manihot esculenta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 mutant enzyme G113S Manihot esculenta
5.6
-
 wild-type enzyme Manihot esculenta

pH Range

pH Minimum pH Maximum Comment Organism
4.5 6.5 pH 4.5: about 65% of maximal activity of mutant enzyme G113S, about 60% of maximal activity of wild-type enzyme, pH 6.5: about 50% of maximal activity of mutant enzyme G113S, about 55% of maximal activity of wild-type enzyme Manihot esculenta

Cofactor

Cofactor Comment Organism Structure
additional information FAD-independent Manihot esculenta