Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | addition of 1 mM thiamine diphosphate to the standard assay causes only a minimal stimulation (8%) | Aromatoleum evansii |
Cloned (Comment) | Organism |
---|---|
the boxC gene is expressed in a recombinant Escherichia coli strain as a fusion protein with maltose binding protein (BoxCmal) | Aromatoleum evansii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | acetoacetyl-CoA (0.2 mM), a potential inhibitor of enoyl-CoA hydratase, has no impact on enzyme activity of BoxCmal. Crotonyl-CoA (0.2 mM), a potential substrate of enoyl-CoA hydratase (crotonase), is neither converted to 3-hydroxybutyryl-CoA nor does it inhibit the standard assay | Aromatoleum evansii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
2,3-dihydro-2,3-dihydroxybenzoyl-CoA | - |
Aromatoleum evansii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the purified protein does not require divalent metals or any cosubstrates or coenzymes for activity | Aromatoleum evansii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
2 * 60000, SDS-PAGE | Aromatoleum evansii |
120000 | - |
gel filtration | Aromatoleum evansii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-dihydro-2,3-dihydroxybenzoyl-CoA + H2O | Aromatoleum evansii | the enzyme is involved in the aerobic benzoyl-CoA catabolic pathway. Benzoyl-CoA is oxidized to 2,3-dihydro-2,3-dihydroxybenzoyl-CoA (benzoyl-CoA dihydrodiol) by benzoyl-CoA oxygenase/reductase BoxBA in the presence of molecular oxygen. The next, ring cleaving step is catalysed by BoxC | 3,4-dehydroadipyl-CoA semialdehyde + formate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aromatoleum evansii | Q84HH6 | - |
- |
Purification (Comment) | Organism |
---|---|
wild type and recombinant proteins | Aromatoleum evansii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:benzoate-grown cell | - |
Aromatoleum evansii | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.9 | - |
- |
Aromatoleum evansii |
Storage Stability | Organism |
---|---|
-20°C, the protein can be stored without appreciable loss of activity for months in the presence of 10% (v/v) glycerol | Aromatoleum evansii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-dihydro-2,3-dihydroxybenzoyl-CoA + H2O | the enzyme is involved in the aerobic benzoyl-CoA catabolic pathway. Benzoyl-CoA is oxidized to 2,3-dihydro-2,3-dihydroxybenzoyl-CoA (benzoyl-CoA dihydrodiol) by benzoyl-CoA oxygenase/reductase BoxBA in the presence of molecular oxygen. The next, ring cleaving step is catalysed by BoxC | Aromatoleum evansii | 3,4-dehydroadipyl-CoA semialdehyde + formate | - |
? | |
2,3-dihydro-2,3-dihydroxybenzoyl-CoA + H2O | NADPH and semicarbazide are analysed directly by NMR spectroscopy and mass spectrometry. The purified protein does not require molecular oxygen for activity | Aromatoleum evansii | 3,4-dehydroadipyl-CoA semialdehyde + formate | - |
? | |
additional information | no activity with crotonyl-CoA | Aromatoleum evansii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 60000, SDS-PAGE | Aromatoleum evansii |
Synonyms | Comment | Organism |
---|---|---|
benzoyl-CoA oxidation component C | - |
Aromatoleum evansii |
BoxC | - |
Aromatoleum evansii |
dihydrodiol transforming enzyme | - |
Aromatoleum evansii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
20 | - |
2,3-dihydro-2,3-dihydroxybenzoyl-CoA | - |
Aromatoleum evansii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Aromatoleum evansii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 11 | half maximal activity at pH 7 and pH 11 | Aromatoleum evansii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the purified protein does not require divalent metals or any cosubstrates or coenzymes for activity | Aromatoleum evansii |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Aromatoleum evansii | - |
- |
5.6 |