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Literature summary for 4.1.2.4 extracted from

  • Ohshida, T.; Hayashi, J.; Satomura, T.; Kawakami, R.; Ohshima, T.; Sakuraba, H.
    First characterization of extremely halophilic 2-deoxy-D-ribose-5-phosphate aldolase (2016), Protein Expr. Purif., 126, 62-68 .
    View publication on PubMed
Search for more literature for 4.1.2.4

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli Haloarcula japonica

General Stability

General Stability Organism
the enzyme is highly resistant to a high concentration of acetaldehyde and retains about 35% of its initial activity after 5-h exposure to 300 mM acetaldehyde at 25°C Haloarcula japonica
the recombinant enzyme is stable in a buffer containing 2 M NaCl Haloarcula japonica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.12
-
 2-deoxy-D-ribose 5-phosphate pH and temperature not specified in the publication Haloarcula japonica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 SDS-PAGE Haloarcula japonica
55500
-
 gel filtration Haloarcula japonica

Organic Solvent Stability

Organic Solvent Comment Organism
acetonitrile 50%, the enzyme retains more than 80% of its activity after incubation for 50 min Haloarcula japonica
dimethyl sulfoxide 50%, the enzyme retains more than 80% of its activity after incubation for 50 min Haloarcula japonica
Ethanol 20%, 25°C, 10 min, the enzyme retains its activity Haloarcula japonica
Methanol 30%, 25°C, 10 min, the enzyme retains its activity Haloarcula japonica

Organism

Organism UniProt Comment Textmining
Haloarcula japonica A0A1Q2T258
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Haloarcula japonica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-ribose 5-phosphate
-
 Haloarcula japonica D-glyceraldehyde 3-phosphate + acetaldehyde
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 29000, SDS-PAGE Haloarcula japonica

Synonyms

Synonyms Comment Organism
2-deoxy-D-ribose-5-phosphate aldolase
-
 Haloarcula japonica
DERA
-
 Haloarcula japonica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Haloarcula japonica

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40
-
 40°C: about 60% of maximal activity Haloarcula japonica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 pH 6.3-12.3, 30 min, enzyme shows no loss of activity Haloarcula japonica
70
-
 10 min, more than 90% of the activity is retained Haloarcula japonica
80
-
 10 min, almost complete loss of activity Haloarcula japonica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4
-
-
 Haloarcula japonica