Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21(DE3) | Rhodococcus erythropolis |
Protein Variants | Comment | Organism |
---|---|---|
C47A | the mutant enzyme is highly sensitive to crotonaldehyde and acetaldehyde | Rhodococcus erythropolis |
C47G | the mutant enzyme is highly sensitive to crotonaldehyde and acetaldehyde | Rhodococcus erythropolis |
C47L | the mutant enzyme shows no loss in stereoselectivity, the mutant is fully resistant to inhibition by crotonaldehyde | Rhodococcus erythropolis |
C47M | the mutant is both, the most acetaldehyde resistant variant and well performing on stereoselectivity, it is less resistant to crotonaldehyde than mutant C47M | Rhodococcus erythropolis |
C47S | although serine is similar to cysteine, this variant is ten times more stable against acetaldehyde than the wildtype | Rhodococcus erythropolis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetaldehyde | - |
Rhodococcus erythropolis | |
crotonaldehyde | the enzyme produces crotonaldehyde as side reaction from acetaldehyde which is then an irreversible inhibitor forming a covalent Michael-adduct within the active site in particular with cysteine 47 | Rhodococcus erythropolis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus erythropolis | C0ZUQ6 | - |
- |
Rhodococcus erythropolis NBRC 100887 | C0ZUQ6 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
2-deoxy-D-ribose-5-phosphate aldolase | - |
Rhodococcus erythropolis |
DeoC | - |
Rhodococcus erythropolis |
DERA | - |
Rhodococcus erythropolis |