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Literature summary for 4.1.2.27 extracted from
- Characterization of homologous sphingosine-1-phosphate lyase isoforms in the bacterial pathogen Burkholderia pseudomallei (2017), J. Lipid Res., 58, 137-150 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Burkholderia pseudomallei |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapor diffusion method, using 20% (w/v) polypropylene glycol 400 and 10% (v/v) 1-propanol | Burkholderia pseudomallei |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K271A | inactive | Burkholderia pseudomallei |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0194 | - |
(2E)-hexadecenal | at pH 7.5 and 37°C | Burkholderia pseudomallei |  |
| 0.0482 | - |
sphinganine 1-phosphate | at pH 7.5 and 37°C | Burkholderia pseudomallei | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sphinganine 1-phosphate | Burkholderia pseudomallei | - |
phosphoethanolamine + palmitaldehyde | - |
ir | |
| sphinganine 1-phosphate | Burkholderia pseudomallei K96243 | - |
phosphoethanolamine + palmitaldehyde | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Burkholderia pseudomallei | Q63IP8 | - |
- |
| Burkholderia pseudomallei K96243 | Q63IP8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA resin column chromatography and Superdex S-200 gel filtration | Burkholderia pseudomallei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E)-hexadecenal + semicarbazide | - |
Burkholderia pseudomallei | (2E)-hexadecenal semicarbazone | - |
? | |
| (2E)-hexadecenal + semicarbazide | - |
Burkholderia pseudomallei K96243 | (2E)-hexadecenal semicarbazone | - |
? | |
| D-erythrosphingosine | 8% activity compared to sphinganine 1-phosphate | Burkholderia pseudomallei | ? | - |
? | |
| D-erythrosphingosine | 8% activity compared to sphinganine 1-phosphate | Burkholderia pseudomallei K96243 | ? | - |
? | |
| sphinganine 1-phosphate | - |
Burkholderia pseudomallei | phosphoethanolamine + palmitaldehyde | - |
ir | |
| sphinganine 1-phosphate | - |
Burkholderia pseudomallei K96243 | phosphoethanolamine + palmitaldehyde | - |
ir | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| S1P lyase | - |
Burkholderia pseudomallei |
| S1PL | - |
Burkholderia pseudomallei |
| S1PL2021 | - |
Burkholderia pseudomallei |
| sphingosine-1-phosphate lyase | - |
Burkholderia pseudomallei |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
sphinganine 1-phosphate | at pH 7.5 and 37°C | Burkholderia pseudomallei | |
| 0.22 | - |
(2E)-hexadecenal | at pH 7.5 and 37°C | Burkholderia pseudomallei | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Burkholderia pseudomallei | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.32 | - |
sphinganine 1-phosphate | at pH 7.5 and 37°C | Burkholderia pseudomallei | |
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Release 2023.1 (January 2023)
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