Literature summary for 4.1.2.17 extracted from

Jaafar, N.R.; Littler, D.; Beddoe, T.; Rossjohn, J.; Illias, R.M.; Mahadi, N.M.; Mackeen, M.M.; Murad, A.M.; Abu Bakar, F.D.
Crystal structure of fuculose aldolase from the antarctic psychrophilic yeast Glaciozyma antarctica PI12 (2016), Acta Crystallogr. Sect. F, 72, 831-839 .

Search for more literature for 4.1.2.17

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Glaciozyma antarctica

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 0.1 M Tris pH 8.0, 25% PEG 8000, 0.25 M MgCl2	Glaciozyma antarctica

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
120000	-	gel filtration	Glaciozyma antarctica

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-fuculose 1-phosphate	Glaciozyma antarctica	-	glycerone phosphate + (S)-lactaldehyde	-	r
L-fuculose 1-phosphate	Glaciozyma antarctica PI12	-	glycerone phosphate + (S)-lactaldehyde	-	r

Organism

Organism	UniProt	Comment	Textmining
Glaciozyma antarctica	-	-	-
Glaciozyma antarctica PI12	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA agarose column chromatography, HiTrap Q column chromatography, and Superdex 200 gel filtration	Glaciozyma antarctica

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-fuculose 1-phosphate	-	Glaciozyma antarctica	glycerone phosphate + (S)-lactaldehyde	-	r
L-fuculose 1-phosphate	-	Glaciozyma antarctica PI12	glycerone phosphate + (S)-lactaldehyde	-	r

Subunits

Subunits	Comment	Organism
homotetramer	4 * 30000, SDS-PAGE	Glaciozyma antarctica
homotetramer	4 * 30688, calculated from amino acid sequence	Glaciozyma antarctica

Synonyms

Synonyms	Comment	Organism
FucA	-	Glaciozyma antarctica
fuculose aldolase	-	Glaciozyma antarctica
fuculose-1-phosphate aldolase	-	Glaciozyma antarctica

pI Value

Organism	Comment	pI Value Maximum	pI Value
Glaciozyma antarctica	calculated from amino acid sequence	-	6

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Release 2023.1 (January 2023)