Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Lupinus angustifolius |
expression in Escherichia coli | Sophora flavescens |
expression in Escherichia coli | Echinosophora koreensis |
Protein Variants | Comment | Organism |
---|---|---|
F344H | mutation in conserved amino acid, 41fold increase in Km value for L-lysine, 1.5fold for L-ornithine | Lupinus angustifolius |
F344Y | mutation in conserved amino acid, 2.2fold increase in Km value for L-lysine, 1.5fold for L-ornithine | Lupinus angustifolius |
M341L | mutation in conserved amino acid, minor changes in Km values | Lupinus angustifolius |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-difluoromethylornithine | inhibitor of ornithine decarboxylase, inhibitory | Lupinus angustifolius | |
L-ornithine | competitive | Echinosophora koreensis | |
L-ornithine | competitive | Lupinus angustifolius | |
L-ornithine | competitive | Sophora flavescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.04 | - |
L-ornithine | mutant M341L, pH 7.5, 37°C | Lupinus angustifolius | |
1.05 | - |
L-ornithine | wild-type, pH 7.5, 37°C | Lupinus angustifolius | |
1.32 | - |
L-ornithine | pH 7.5, 37°C | Echinosophora koreensis | |
1.53 | - |
L-ornithine | pH 7.5, 37°C | Sophora flavescens | |
1.53 | - |
L-ornithine | mutant F344H, pH 7.5, 37°C | Lupinus angustifolius | |
1.59 | - |
L-ornithine | mutant F344Y, pH 7.5, 37°C | Lupinus angustifolius | |
2.1 | - |
L-lysine | pH 7.5, 37°C | Sophora flavescens | |
2.37 | - |
L-lysine | wild-type, pH 7.5, 37°C | Lupinus angustifolius | |
2.49 | - |
L-lysine | mutant M341L, pH 7.5, 37°C | Lupinus angustifolius | |
3.85 | - |
L-lysine | pH 7.5, 37°C | Echinosophora koreensis | |
5.26 | - |
L-lysine | mutant F344Y, pH 7.5, 37°C | Lupinus angustifolius | |
97.8 | - |
L-lysine | mutant F344H, pH 7.5, 37°C | Lupinus angustifolius |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plastid | - |
Lupinus angustifolius | 9536 | - |
plastid | - |
Sophora flavescens | 9536 | - |
plastid | - |
Echinosophora koreensis | 9536 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
x * 47000, SDS-PAGE | Lupinus angustifolius |
49000 | - |
x * 49000, SDS-PAGE | Sophora flavescens |
49000 | - |
x * 49000, SDS-PAGE | Echinosophora koreensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Echinosophora koreensis | F5HPS1 | - |
- |
Lupinus angustifolius | F5HPR7 | - |
- |
Sophora flavescens | F5HPS0 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-ornithine = putrescine + CO2 | decarboxylation of L-ornithine and L-lysine is catalyzed with similar efficiency | Lupinus angustifolius |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | in bitter cultivar, highest expression in young leaf | Lupinus angustifolius | - |
additional information | no detectable expression in sweet cultivar | Lupinus angustifolius | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine | the ratio of catalytic efficiency with L-Lys to efficiency with L-Orn is 0.5 in wild type | Lupinus angustifolius | cadaverine + CO2 | - |
? | |
L-lysine | the ratio of catalytic efficiency with L-Lys to efficiency with L-Orn is 1.03 | Echinosophora koreensis | cadaverine + CO2 | - |
? | |
L-lysine | the ratio of catalytic efficiency with L-Lys to efficiency with L-Orn is 1.47 | Sophora flavescens | cadaverine + CO2 | - |
? | |
L-ornithine | the ratio of catalytic efficiency with L-Lys to efficiency with L-Orn is 0.5 in wild type | Lupinus angustifolius | putrescine + CO2 | - |
? | |
L-ornithine | the ratio of catalytic efficiency with L-Lys to efficiency with L-Orn is 1.03 | Echinosophora koreensis | putrescine + CO2 | - |
? | |
L-ornithine | the ratio of catalytic efficiency with L-Lys to efficiency with L-Orn is 1.47 | Sophora flavescens | putrescine + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 47000, SDS-PAGE | Lupinus angustifolius |
? | x * 49000, SDS-PAGE | Sophora flavescens |
? | x * 49000, SDS-PAGE | Echinosophora koreensis |
Synonyms | Comment | Organism |
---|---|---|
L/ODC | - |
Lupinus angustifolius |
L/ODC | - |
Sophora flavescens |
L/ODC | - |
Echinosophora koreensis |
LDC | - |
Lupinus angustifolius |
LDC | - |
Sophora flavescens |
LDC | - |
Echinosophora koreensis |
Lys/Orn decarboxylase | - |
Lupinus angustifolius |
Lys/Orn decarboxylase | - |
Sophora flavescens |
Lys/Orn decarboxylase | - |
Echinosophora koreensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.29 | - |
L-ornithine | mutant M341L, pH 7.5, 37°C | Lupinus angustifolius | |
0.4 | - |
L-ornithine | mutant F344Y, pH 7.5, 37°C | Lupinus angustifolius | |
0.73 | - |
L-ornithine | pH 7.5, 37°C | Echinosophora koreensis | |
0.91 | - |
L-ornithine | wild-type, pH 7.5, 37°C | Lupinus angustifolius | |
1.16 | - |
L-ornithine | pH 7.5, 37°C | Sophora flavescens | |
1.18 | - |
L-lysine | wild-type, pH 7.5, 37°C | Lupinus angustifolius | |
1.91 | - |
L-lysine | pH 7.5, 37°C | Echinosophora koreensis | |
2.3 | - |
L-ornithine | mutant F344H, pH 7.5, 37°C | Lupinus angustifolius | |
2.32 | - |
L-lysine | pH 7.5, 37°C | Sophora flavescens | |
3.88 | - |
L-lysine | mutant M341L, pH 7.5, 37°C | Lupinus angustifolius | |
6.33 | - |
L-lysine | mutant F344Y, pH 7.5, 37°C | Lupinus angustifolius | |
143 | - |
L-lysine | mutant F344H, pH 7.5, 37°C | Lupinus angustifolius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Lupinus angustifolius |
7.5 | - |
- |
Sophora flavescens |
7.5 | - |
- |
Echinosophora koreensis |
General Information | Comment | Organism |
---|---|---|
physiological function | transgenic tobacco suspension cells and hairy roots expressing lysine decarboxylase produce enhanced levels of cadaverine-derived alkaloids, and transgenic Arabidopsis plants produce enhanced levels of cadaverine, indicating the involvement of this enzyme in lysine decarboxylation to form cadaverine. Enzyme is involved in the decarboxylation of L-Lys, which is the first step of quinolizidine alkaloid biosynthesis in quinolizidine alkaloid-producing plants | Lupinus angustifolius |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.454 | - |
L-ornithine | pH 7.5, 37°C | Echinosophora koreensis | |
0.469 | - |
L-lysine | pH 7.5, 37°C | Echinosophora koreensis | |
0.755 | - |
L-ornithine | pH 7.5, 37°C | Sophora flavescens | |
1.108 | - |
L-lysine | pH 7.5, 37°C | Sophora flavescens | |
253 | - |
L-ornithine | mutant F344Y, pH 7.5, 37°C | Lupinus angustifolius | |
279 | - |
L-ornithine | mutant M341L, pH 7.5, 37°C | Lupinus angustifolius | |
433 | - |
L-lysine | wild-type, pH 7.5, 37°C | Lupinus angustifolius | |
859 | - |
L-ornithine | wild-type, pH 7.5, 37°C | Lupinus angustifolius | |
1203 | - |
L-lysine | mutant F344Y, pH 7.5, 37°C | Lupinus angustifolius | |
1471 | - |
L-lysine | mutant F344H, pH 7.5, 37°C | Lupinus angustifolius | |
1496 | - |
L-ornithine | mutant F344H, pH 7.5, 37°C | Lupinus angustifolius | |
1558 | - |
L-lysine | mutant M341L, pH 7.5, 37°C | Lupinus angustifolius |