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Literature summary for 4.1.1.97 extracted from

  • French, J.B.; Ealick, S.E.
    Structural and mechanistic studies on Klebsiella pneumoniae 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase (2010), J. Biol. Chem., 285, 35446-35454.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.97

Cloned(Commentary)

Cloned (Comment) Organism
-
 Klebsiella pneumoniae

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapor-diffusion method at 18°C Klebsiella pneumoniae

Protein Variants

Protein Variants Comment Organism
Q88E the maximal rate of this mutant is 43fold slower than that of the native rate, whereas the measured Km for the mutant is similar to that of the native enzyme, the kcat/Km value is about 50fold lower than the wild-type value Klebsiella pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
allopurinol competitive inhibitor. The inhibitor disrupts the necessary organization of the active site Klebsiella pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.151
-
 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate mutant enzyme Q88E, pH and temperature not specified in the publication Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate Klebsiella pneumoniae the enzyme is part of the pathway from urate to (S)-allantoin (S)-allantoin + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae A6T925
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate
-
 Klebsiella pneumoniae (S)-allantoin + CO2
-
 ?
5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate the enzyme is part of the pathway from urate to (S)-allantoin Klebsiella pneumoniae (S)-allantoin + CO2
-
 ?

Synonyms

Synonyms Comment Organism
hpxQ
-
 Klebsiella pneumoniae
OHCU decarboxylase
-
 Klebsiella pneumoniae
PRHOXNB
-
 Klebsiella pneumoniae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
122
-
 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate mutant enzyme Q88E, pH and temperature not specified in the publication Klebsiella pneumoniae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.03
-
 allopurinol mutant enzyme Q88E, pH and temperature not specified in the publication Klebsiella pneumoniae

General Information

General Information Comment Organism
physiological function the enzyme is part of the pathway from urate to (S)-allantoin Klebsiella pneumoniae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
19
-
 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate mutant enzyme Q88E, pH and temperature not specified in the publication Klebsiella pneumoniae
970
-
 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate wild-type enzyme, pH and temperature not specified in the publication Klebsiella pneumoniae