General Stability | Organism |
---|---|
loss of stabililty by freezing and thawing | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al(NO3)3 | - |
Pseudomonas sp. | |
BaCl2 | - |
Pseudomonas sp. | |
CN- | - |
Pseudomonas sp. | |
CoCl2 | - |
Pseudomonas sp. | |
EDTA | - |
Pseudomonas sp. | |
Fe(NO3)3 | - |
Pseudomonas sp. | |
HgCl2 | - |
Pseudomonas sp. | |
NiCl2 | - |
Pseudomonas sp. | |
p-chloromercuribenzoate | - |
Pseudomonas sp. | |
ZnSO4 | - |
Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
acetylene dicarboxylate | 23°C, pH 7.3 | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylene dicarboxylate | Pseudomonas sp. | - |
pyruvate + CO2 | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
no activity in Clostridium cylindrosporum | - |
- |
- |
no activity in Clostridium kluyveri | - |
- |
- |
no activity in Escherichia coli | - |
- |
- |
no activity in Pseudomonas fluorescens | - |
- |
- |
no activity in Rattus norvegicus | - |
- |
- |
Pseudomonas sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Pseudomonas sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
463 | - |
- |
Pseudomonas sp. |
Storage Stability | Organism |
---|---|
-20°C, 0.005 mM 2-mercaptoethanol, 10 days, less than 10% loss of activity | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylene dicarboxylate | - |
Pseudomonas sp. | pyruvate + CO2 | - |
ir |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
enzyme assay at | Pseudomonas sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
- |
Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | 7.8 | - |
Pseudomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | - |
Pseudomonas sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
highest stability at 3°C for 17 h | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement | Pseudomonas sp. |