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Literature summary for 4.1.1.76 extracted from
- Screening, cloning, expression, and purification of an acidic arylmalonate decarboxylase from Enterobacter cloacae KU1313 (2008), Appl. Microbiol. Biotechnol., 78, 793-799.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Enterobacter cloacae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
SDS-PAGE | Enterobacter cloacae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-2-(2-naphthyl)propionate | Enterobacter cloacae | - |
? | - |
? |  |
| (R)-2-(2-naphthyl)propionate | Enterobacter cloacae KU1313 | - |
? | - |
? | |
| (R)-2-(2-thienyl)propionate | Enterobacter cloacae | - |
? | - |
? | |
| (R)-2-(2-thienyl)propionate | Enterobacter cloacae KU1313 | - |
? | - |
? | |
| (R)-2-phenylpropionate | Enterobacter cloacae | - |
? | - |
? | |
| (R)-2-phenylpropionate | Enterobacter cloacae KU1313 | - |
? | - |
? | |
| (S)-2-(2-naphtyl)propionate | Enterobacter cloacae | - |
? | - |
? | |
| (S)-2-(2-naphtyl)propionate | Enterobacter cloacae KU1313 | - |
? | - |
? | |
| (S)-2-(2-thienyl)propionate | Enterobacter cloacae | - |
? | - |
? | |
| (S)-2-(2-thienyl)propionate | Enterobacter cloacae KU1313 | - |
? | - |
? | |
| (S)-2-phenylpropionate | Enterobacter cloacae | - |
? | - |
? | |
| 2-methyl-alpha-phenylmalonate | Enterobacter cloacae | - |
? + CO2 | - |
? | |
| 2-phenylmalonate | Enterobacter cloacae | - |
phenylacetate + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterobacter cloacae | - |
KU1313 | - |
| Enterobacter cloacae KU1313 | - |
KU1313 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Enterobacter cloacae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 381 | - |
butyl-Toyopearl, last purification step | Enterobacter cloacae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| enzyme can be stored at 4°C for over 3 months. | Enterobacter cloacae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-2-(2-naphthyl)propionate | - |
Enterobacter cloacae | ? | - |
? | |
| (R)-2-(2-naphthyl)propionate | - |
Enterobacter cloacae KU1313 | ? | - |
? | |
| (R)-2-(2-thienyl)propionate | - |
Enterobacter cloacae | ? | - |
? | |
| (R)-2-(2-thienyl)propionate | - |
Enterobacter cloacae KU1313 | ? | - |
? | |
| (R)-2-phenylpropionate | - |
Enterobacter cloacae | ? | - |
? | |
| (R)-2-phenylpropionate | - |
Enterobacter cloacae KU1313 | ? | - |
? | |
| (S)-2-(2-naphtyl)propionate | - |
Enterobacter cloacae | ? | - |
? | |
| (S)-2-(2-naphtyl)propionate | - |
Enterobacter cloacae KU1313 | ? | - |
? | |
| (S)-2-(2-thienyl)propionate | - |
Enterobacter cloacae | ? | - |
? | |
| (S)-2-(2-thienyl)propionate | - |
Enterobacter cloacae KU1313 | ? | - |
? | |
| (S)-2-phenylpropionate | - |
Enterobacter cloacae | ? | - |
? | |
| 2-methyl-alpha-phenylmalonate | - |
Enterobacter cloacae | ? + CO2 | - |
? | |
| 2-phenylmalonate | - |
Enterobacter cloacae | phenylacetate + CO2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AMDase | - |
Enterobacter cloacae |
| Arylmalonate decarboxylase | - |
Enterobacter cloacae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
AMDase activity reaches its maximum at approximately 35°C. Optimum temperature for AMDase activity is examined in the range of 25-50°C at pH 6.0 with phenylmalonate as the substrate. It decreases to 40% of the maximum at 45°C | Enterobacter cloacae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 50 | AMDase activity reaches its maximum at approximately 35°C. Optimum temperature for AMDase activity is examined in the range of 25-50°C at pH 6.0 with phenylmalonate as the substrate | Enterobacter cloacae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 45 | thermal stability of the enzyme is examined in the range of 30-45°C by measuring the residual activity after a 10 min incubation at specified temperatures using phenylmalonate as the substrate. The enzyme retains 85% activity after incubation at 30°C. The activity dramatically decreases after incubation at 35°C, and it is almost negligible after incubation at 45°C | Enterobacter cloacae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
maximum activity. pH-activity profiles are different from those of other AMDases | Enterobacter cloacae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 11 | no loss of enzyme activity is detected after incubation at 25°C for 60 min in the pH range of 5-11 | Enterobacter cloacae |
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