Protein Variants | Comment | Organism |
---|---|---|
C188S | activity with methyl(2-thienyl)malonic acid is 0.7% of wild-type activity, activity with methyl(2-naphthyl)malonic acid is 2.8% of the wild-type activity, with formation of the opposite enantiomer | Bordetella bronchiseptica |
G74C | activity with methyl(2-thienyl)malonic acid is 0.79% of wild-type activity, activity with methyl(2-naphthyl)malonic acid is 0.44% of the wild-type activity, with formation of the opposite enantiomer | Bordetella bronchiseptica |
G74C/C188S | activity with methyl(2-thienyl)malonic acid is 0.42% of wild-type activity, activity with methyl(2-naphthyl)malonic acid is 0.13% of the wild-type activity, with formation of the opposite enantiomer | Bordetella bronchiseptica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.43 | - |
methyl(2-naphthyl)malonic acid | wild-type enzyme | Bordetella bronchiseptica | |
1.25 | - |
methyl(2-naphthyl)malonic acid | mutant enzyme G74C/C188S | Bordetella bronchiseptica | |
1.26 | - |
methyl(2-naphthyl)malonic acid | mutant enzyme C188S | Bordetella bronchiseptica | |
1.56 | - |
methyl(2-naphthyl)malonic acid | mutant enzyme G74C | Bordetella bronchiseptica | |
5.03 | - |
methyl(2-thienyl)malonic acid | mutant enzyme G74C/C188S | Bordetella bronchiseptica | |
6.24 | - |
methyl(2-thienyl)malonic acid | mutant enzyme G74C | Bordetella bronchiseptica | |
12.5 | - |
methyl(2-thienyl)malonic acid | wild-type enzyme | Bordetella bronchiseptica | |
14.5 | - |
methyl(2-thienyl)malonic acid | mutant enzyme C188S | Bordetella bronchiseptica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bordetella bronchiseptica | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl(2-naphthyl)malonic acid | - |
Bordetella bronchiseptica | (2R)-2-(2-naphthyl)propanoic acid + CO2 | - |
? | |
methyl(2-thienyl)malonic acid | - |
Bordetella bronchiseptica | (2S)-2-(2-thienyl)propanoic acid + CO2 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
methyl(2-naphthyl)malonic acid | mutant enzyme G74C/C188S | Bordetella bronchiseptica | |
0.34 | - |
methyl(2-thienyl)malonic acid | mutant enzyme G74C/C188S | Bordetella bronchiseptica | |
0.5 | - |
methyl(2-naphthyl)malonic acid | mutant enzyme G74C | Bordetella bronchiseptica | |
0.79 | - |
methyl(2-thienyl)malonic acid | mutant enzyme G74C | Bordetella bronchiseptica | |
1.63 | - |
methyl(2-thienyl)malonic acid | mutant enzyme C188S | Bordetella bronchiseptica | |
2.54 | - |
methyl(2-naphthyl)malonic acid | mutant enzyme C188S | Bordetella bronchiseptica | |
31 | - |
methyl(2-naphthyl)malonic acid | wild-type enzyme | Bordetella bronchiseptica | |
200 | - |
methyl(2-thienyl)malonic acid | wild-type enzyme | Bordetella bronchiseptica |