Literature summary for 4.1.1.7 extracted from

Kara, S.; Long, W.S.; Berheide, M.; Peper, S.; Niemeyer, B.; Liese, A.
Influence of reaction conditions on the enantioselectivity of biocatalyzed C-C bond formations under high pressure conditions (2011), J. Biotechnol., 152, 87-92.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli SG13009 cells	Pseudomonas putida

Protein Variants

Protein Variants	Comment	Organism
A460I	the mutant exhibits an increased (R)-2-hydroxypropiophenone selectivity	Pseudomonas putida
A460I/F464I	the mutant exhibits an increased (R)-2-hydroxypropiophenone selectivity	Pseudomonas putida
F464I	the mutant exhibits an increased (R)-2-hydroxypropiophenone selectivity	Pseudomonas putida

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
benzoylformate	Pseudomonas putida	-	benzaldehyde + CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Q-Sepharose column chromatography and Superdex 200 gel filtration	Pseudomonas putida

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
benzaldehyde + acetaldehyde	reactions performed at high benzaldehyde concentrations and at high hydrostatic pressures show an increase in (R)-2-hydroxypropiophenone ((R)-2-HPP) formation catalyzed by BFD variants F464I, A460I, and A460I/F464I. For BFD mutant A460I/F464I there is an increase in the ee of (R)-2-HPP up to 80%, whereas at atmospheric conditions this variant synthesizes (R)-2-HPP with an ee of only 50%. Alkaline conditions (up to pH 8.5) and high hydrostatic pressures result in an increase of (R)-2-HPP synthesis, especially in the case of variant A460I and F464I	Pseudomonas putida	(R)-2-hydroxypropiophenone	-	?
benzoylformate	-	Pseudomonas putida	benzaldehyde + CO2	-	?

Subunits

Subunits	Comment	Organism
homotetramer	-	Pseudomonas putida

Synonyms

Synonyms	Comment	Organism
BFD	-	Pseudomonas putida

Cofactor

Cofactor	Comment	Organism	Structure
thiamine diphosphate	dependent on	Pseudomonas putida

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Release 2023.1 (January 2023)