Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Plasmodium knowlesi |
Protein Variants | Comment | Organism |
---|---|---|
D139A | mutation prevents processing of the proenzyme | Plasmodium knowlesi |
D139N | mutation prevents processing of the proenzyme | Plasmodium knowlesi |
G307A | mutation eliminates processing | Plasmodium knowlesi |
G307P | mutation eliminates processing | Plasmodium knowlesi |
H195A | mutation produces significant levels of enzyme processing, although the level of the beta-subunit is reduced compared with wild-type | Plasmodium knowlesi |
H195A/H198A | mutation prevents processing of the proenzyme | Plasmodium knowlesi |
H198A | mutation prevents processing of the proenzyme | Plasmodium knowlesi |
S*308A | mutation eliminates processing | Plasmodium knowlesi |
S*308T | mutation eliminates processing | Plasmodium knowlesi |
S309A | proenzyme is processed comparably to wild-type, modest reductions in catalytic activity | Plasmodium knowlesi |
S309T | proenzyme is processed comparably to wild-type, modest reductions in catalytic activity | Plasmodium knowlesi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium knowlesi | B3L2V1 | - |
- |
Plasmodium knowlesi H | B3L2V1 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
acylation | the pyruvoyl moiety is detected at the N-termini of alpha-subunits | Plasmodium knowlesi |
proteolytic modification | the post-translational processing is inhibited by the serine protease inhibitor, phenylmethylsulfonyl fluoride. Residues Asp139, His198, and Ser308 are all essential for endoproteolytic processing of PSD, which occurs in cis. Within the GS(S/T) motif found in all PSDs, the Gly307 residue is also essential, but the Ser/Thr309 is nonessential | Plasmodium knowlesi |
Synonyms | Comment | Organism |
---|---|---|
PKH_072580 | - |
Plasmodium knowlesi |
PSD | - |
Plasmodium knowlesi |