Activating Compound | Comment | Organism | Structure |
---|---|---|---|
putrescine | no requirement | Saccharolobus solfataricus | |
pyruvate | covalently linked prosthetic group | Saccharolobus solfataricus | |
pyruvate | contains approximately 0.8 mol pyruvate per mol of enzyme | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
methylglyoxal bis(guanylhydrazone) | competitive | Saccharolobus solfataricus | |
NaCNBH3 | in presence of substrate or product | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.96 | - |
S-adenosylmethionine | - |
Saccharolobus solfataricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement for a divalent metal ion | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
1 * 32000, SDS-PAGE | Saccharolobus solfataricus |
36000 | - |
gel filtration | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine | Saccharolobus solfataricus | major regulatory enzyme in polyamine biosynthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.012 | - |
- |
Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine | - |
Saccharolobus solfataricus | (5-deoxy-5-adenosyl)(3-aminopropyl)methylsulfonium salt + CO2 | - |
? | |
S-adenosyl-L-methionine | major regulatory enzyme in polyamine biosynthesis | Saccharolobus solfataricus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 32000, SDS-PAGE | Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 90 | 50°C: about 45% of maximal activity, 90°C: about 60% of maximal activity | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
16 h, stable | Saccharolobus solfataricus |
70 | - |
16h, 20% loss of activity | Saccharolobus solfataricus |
100 | - |
1 h, 80% loss of activity | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Saccharolobus solfataricus |