Cloned (Comment) | Organism |
---|---|
mutant plasmid is transformed into the phosphoenolpyruvate carboxykinase-deficient yeast strain PUK-3B (MATalpha pck ura3) | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
S252A | side directed mutagenesis | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | MnATP2- as substrate, for S252A mutant Km above 3000 microM | Saccharomyces cerevisiae | |
0.026 | - |
oxaloacetate | S252A mutant | Saccharomyces cerevisiae | |
0.03 | - |
oxaloacetate | wild-type | Saccharomyces cerevisiae | |
0.034 | - |
ADP | wild-type | Saccharomyces cerevisiae | |
0.034 | - |
ADP | ADP in form of MnADP- | Saccharomyces cerevisiae | |
0.037 | - |
phosphoenolpyruvate | for the pyruvate kinase-like activity of S252A mutant | Saccharomyces cerevisiae | |
0.067 | - |
ATP | wild-type | Saccharomyces cerevisiae | |
0.067 | - |
ATP | ATP in form of MnATP2- | Saccharomyces cerevisiae | |
0.077 | - |
ADP | for the pyruvate kinase-like activity of S252A mutant | Saccharomyces cerevisiae | |
0.077 | - |
ADP | ADP in form of MnADP- | Saccharomyces cerevisiae | |
0.082 | - |
phosphoenolpyruvate | S252A mutant | Saccharomyces cerevisiae | |
0.122 | - |
oxalacetate | for the oxaloacetate decarboxylase activity of wild-type | Saccharomyces cerevisiae | |
0.132 | - |
ADP | S252A mutant | Saccharomyces cerevisiae | |
0.132 | - |
ADP | ADP in form of MnADP- | Saccharomyces cerevisiae | |
0.135 | - |
phosphoenolpyruvate | for the pyruvate kinase-like activity of wild-type | Saccharomyces cerevisiae | |
0.151 | - |
ADP | for the pyruvate kinase-like activity of wild-type | Saccharomyces cerevisiae | |
0.151 | - |
ADP | ADP in form of MnADP- | Saccharomyces cerevisiae | |
0.307 | - |
phosphoenolpyruvate | wild-type | Saccharomyces cerevisiae | |
5.2 | - |
oxalacetate | for the oxaloacetate decarboxylase activity of S252A mutant | Saccharomyces cerevisiae | |
18 | - |
CO2 | wild-type | Saccharomyces cerevisiae | |
18 | - |
CO2 | S252A mutant | Saccharomyces cerevisiae | |
18 | - |
CO2 | CO2 in form of HCO3- | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P10963 | - |
- |
Purification (Comment) | Organism |
---|---|
mutant phosphoenolpyruvate carboxykinase | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphoenolpyruvate + CO2 | - |
Saccharomyces cerevisiae | ATP + oxaloacetate | - |
r | |
ATP + oxaloacetate | interacts also with GTP, EC 4.1.1.32 | Saccharomyces cerevisiae | ADP + phosphoenolpyruvate + CO2 | - |
r |
Synonyms | Comment | Organism |
---|---|---|
ATP:oxaloacetate carboxy-lyase | transphosphorylating, interacts also with GTP, EC 4.1.1.32 | Saccharomyces cerevisiae |
PEP carboxykinase | - |
Saccharomyces cerevisiae |
phosphoenolpyruvate carboxykinase | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kcat for oxalacetate as a substrate is above 19 1/s for S252A mutant in the phosphoenolpyruvate formation direction | Saccharomyces cerevisiae | |
0.2 | - |
phosphoenolpyruvate | for the pyruvate kinase-like activity of S252A mutant | Saccharomyces cerevisiae | |
0.32 | - |
oxalacetate | for the oxaloacetate decarboxylase activity of wild-type | Saccharomyces cerevisiae | |
0.5 | - |
phosphoenolpyruvate | S252A mutant, apparent kinetic parameters for S252A mutant in the phosphoenolpyruvate carboxylation direction | Saccharomyces cerevisiae | |
0.8 | - |
phosphoenolpyruvate | for the pyruvate kinase-like activity of wild-type | Saccharomyces cerevisiae | |
1 | - |
oxalacetate | for the oxaloacetate decarboxylase activity of S252A mutant | Saccharomyces cerevisiae | |
62 | - |
phosphoenolpyruvate | wild-type, apparent kinetic parameters for wild-type in the phosphoenolpyruvate carboxylation direction | Saccharomyces cerevisiae | |
65 | - |
oxalacetate | wild-type, apparent kinetic parameters for wild-type in the phosphoenolpyruvate formation direction | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharomyces cerevisiae |