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Literature summary for 4.1.1.38 extracted from

  • Chiba, Y.; Kamikawa, R.; Nakada-Tsukui, K.; Saito-Nakano, Y.; Nozaki, T.
    Discovery of PPi-type phosphoenolpyruvate carboxykinase genes in eukaryotes and bacteria (2015), J. Biol. Chem., 290, 23960-23970 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Entamoeba histolytica

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Entamoeba histolytica 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
142000
-
gel filtration Entamoeba histolytica
440000
-
gel filtration Entamoeba histolytica

Organism

Organism UniProt Comment Textmining
Entamoeba histolytica C4LWQ8 isoform PEPCK3
-
Entamoeba histolytica C4LY96 isoform PEPCK1
-
Entamoeba histolytica C4M1E4 isoform PEPCK2
-

Purification (Commentary)

Purification (Comment) Organism
-
Entamoeba histolytica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.9
-
pH 6.5, 37°C Entamoeba histolytica
8.9
-
pH 6.5, 37°C Entamoeba histolytica
23
-
pH 6.5, 37°C Entamoeba histolytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphate + phosphoenolpyruvate + CO2
-
Entamoeba histolytica diphosphate + oxaloacetate
-
r

Subunits

Subunits Comment Organism
monomer 1 * 130000, SDS-PAGE, and trimer Entamoeba histolytica
monomer 1 * 130000, SDS-PAGE, monomer and trimer Entamoeba histolytica
trimer 3 * 130000, SDS-PAGE, and monomer Entamoeba histolytica
trimer 3 * 130000, SDS-PAGE, trimer and monomer Entamoeba histolytica

Synonyms

Synonyms Comment Organism
PEPCK1
-
Entamoeba histolytica
PEPCK2
-
Entamoeba histolytica
PEPCK3
-
Entamoeba histolytica