KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
oxaloacetate | pH 7.2 | Lithobates catesbeianus | |
0.067 | - |
GTP | exchange reaction | Lithobates catesbeianus | |
0.2 | - |
ITP | exchange reaction | Lithobates catesbeianus | |
0.8 | - |
phosphoenolpyruvate | pH 7.2 | Lithobates catesbeianus | |
0.8 | - |
IDP | pH 6.3 | Lithobates catesbeianus | |
0.8 | - |
GDP | at pH 6.3 or at pH 7.2 | Lithobates catesbeianus | |
1 | - |
oxaloacetate | exchange reaction | Lithobates catesbeianus | |
1.6 | - |
GTP | pH 7.2 | Lithobates catesbeianus | |
2.5 | - |
IDP | pH 7.2 | Lithobates catesbeianus | |
4 | - |
ITP | pH 7.2 | Lithobates catesbeianus | |
8 | - |
CO2 | pH 6.3 | Lithobates catesbeianus | |
8 | - |
CO2 | CO2 in form of HCO3- | Lithobates catesbeianus | |
9.6 | - |
phosphoenolpyruvate | pH 6.3 | Lithobates catesbeianus | |
31 | - |
CO2 | exchange reaction | Lithobates catesbeianus | |
31 | - |
CO2 | CO2 in form of HCO3- | Lithobates catesbeianus | |
46 | - |
CO2 | pH 7.2 | Lithobates catesbeianus | |
46 | - |
CO2 | CO2 in form of HCO3- | Lithobates catesbeianus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Lithobates catesbeianus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | can replace Mn2+ in activation of oxaloacetate decarboxylation | Lithobates catesbeianus | |
Mn2+ | absolutely required for phosphoenolpyruvate carboxylation | Lithobates catesbeianus | |
Mn2+ | half-maximal activation: of phosphoenolpyruvate carboxylation at 2 mM, of oxaloacetate decarboxylation at 0.4 mM, of exchange reaction at 8 mM | Lithobates catesbeianus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
glycerol gradient centrifugation | Lithobates catesbeianus |
75000 | - |
1 * 75000, SDS-PAGE | Lithobates catesbeianus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lithobates catesbeianus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Lithobates catesbeianus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Lithobates catesbeianus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP + phosphoenolpyruvate + CO2 | - |
Lithobates catesbeianus | GTP + oxaloacetate | - |
r | |
GTP + oxaloacetate | - |
Lithobates catesbeianus | GDP + phosphoenolpyruvate + CO2 | - |
? | |
IDP + phosphoenolpyruvate + CO2 | - |
Lithobates catesbeianus | ITP + oxaloacetate | - |
? | |
ITP + oxaloacetate | - |
Lithobates catesbeianus | IDP + phosphoenolpyruvate + CO2 | - |
? | |
additional information | catalyzes an exchange reaction between HCO3- and the beta-carboxyl group of oxaloacetate | Lithobates catesbeianus | ? | - |
? | |
additional information | ADP is completely inactive in carboxylation of oxaloacetate | Lithobates catesbeianus | ? | - |
? | |
UDP + phosphoenolpyruvate + CO2 | very low activity | Lithobates catesbeianus | UTP + oxaloacetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 75000, SDS-PAGE | Lithobates catesbeianus |