Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells | Phaeodactylum tricornutum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.169 | - |
HCO3- | at pH 8.0 and 25°C | Phaeodactylum tricornutum | |
0.79 | - |
phosphoenolpyruvate | at pH 8.0 and 25°C | Phaeodactylum tricornutum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Phaeodactylum tricornutum | 5829 | - |
mitochondrion | - |
Phaeodactylum tricornutum | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
117000 | - |
x * 117000, recombinant enzyme, SDS-PAGE | Phaeodactylum tricornutum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + HCO3- | Phaeodactylum tricornutum | - |
phosphate + oxaloacetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phaeodactylum tricornutum | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
41.7 | - |
at pH 8.0 and 25°C | Phaeodactylum tricornutum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + HCO3- | - |
Phaeodactylum tricornutum | phosphate + oxaloacetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 117000, recombinant enzyme, SDS-PAGE | Phaeodactylum tricornutum |
Synonyms | Comment | Organism |
---|---|---|
PEPCase1 | - |
Phaeodactylum tricornutum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 45 | the enzyme remains stable | Phaeodactylum tricornutum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | - |
Phaeodactylum tricornutum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Phaeodactylum tricornutum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
52.7 | - |
phosphoenolpyruvate | at pH 8.0 and 25°C | Phaeodactylum tricornutum |