Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Thermus sp. | |
Asp | - |
Thermus sp. | |
ATP | - |
Thermus sp. | |
fructose 1,6-diphosphate | - |
Thermus sp. | |
glucose 6-phosphate | - |
Thermus sp. | |
GTP | - |
Thermus sp. | |
guanidine hydrochloride | - |
Thermus sp. | |
malate | - |
Thermus sp. | |
phosphate | - |
Thermus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
4 * 100000, SDS-PAGE | Thermus sp. |
400000 | - |
gel filtration | Thermus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
29.4 | - |
- |
Thermus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + CO2 | - |
Thermus sp. | phosphate + oxaloacetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 100000, SDS-PAGE | Thermus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
in presence of 0.3 M acetyl-CoA and 2 mM phosphoenolpyruvate | Thermus sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 80 | about 50% of maximal activity at 50°C and 80°C | Thermus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
half-life: 85 min | Thermus sp. |
90 | - |
half-life: 110 min | Thermus sp. |
95 | - |
half-life: 50 min | Thermus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
at 30°C | Thermus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Thermus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | strongly dependent on the allosteric activator | Thermus sp. |