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Literature summary for 4.1.1.20 extracted from

  • Hu, T.; Wu, D.; Chen, J.; Ding, J.; Jiang, H.; Shen, X.
    The catalytic intermediate stabilized by a "down" active site loop for diaminopimelate decarboxylase from Helicobacter pylori. Enzymatic characterization with crystal structure analysis (2008), J. Biol. Chem., 283, 21284-21293.
    View publication on PubMed
Search for more literature for 4.1.1.20

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21 (DE3) Helicobacter pylori

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method, in 100 mM bicine, pH 8.0, and 1.6 M ammonium sulfate, at 4°C Helicobacter pylori

Protein Variants

Protein Variants Comment Organism
I148A mutant shows 2% catalytic efficiency compared to the wild type enzyme Helicobacter pylori
I148D mutant shows 0.2% catalytic efficiency compared to the wild type enzyme Helicobacter pylori
I148F mutant shows 15% catalytic efficiency compared to the wild type enzyme Helicobacter pylori
I148G mutant shows 0.5% catalytic efficiency compared to the wild type enzyme Helicobacter pylori
I148K mutant shows 2% catalytic efficiency compared to the wild type enzyme Helicobacter pylori
I148L mutant shows 28% catalytic efficiency compared to the wild type enzyme Helicobacter pylori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 meso-2,6-diaminoheptanedioate mutant enzyme I148L, in 100 mM Tris, pH 8.0 Helicobacter pylori
0.11
-
 meso-2,6-diaminoheptanedioate mutant enzyme I148F, in 100 mM Tris, pH 8.0 Helicobacter pylori
0.35
-
 meso-2,6-diaminoheptanedioate mutant enzyme I148A, in 100 mM Tris, pH 8.0 Helicobacter pylori
0.36
-
 meso-2,6-diaminoheptanedioate mutant enzyme I148K, in 100 mM Tris, pH 8.0 Helicobacter pylori
0.36
-
 meso-2,6-diaminoheptanedioate mutant enzyme I148G, in 100 mM Tris, pH 8.0 Helicobacter pylori
0.39
-
 meso-2,6-diaminoheptanedioate wild type enzyme, in 100 mM Tris, pH 8.0 Helicobacter pylori
0.72
-
 meso-2,6-diaminoheptanedioate mutant enzyme I148D, in 100 mM Tris, pH 8.0 Helicobacter pylori

Organism

Organism UniProt Comment Textmining
Helicobacter pylori P56129
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography Helicobacter pylori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
meso-2,6-Diaminoheptanedioate
-
 Helicobacter pylori L-Lysine + CO2
-
 ?

Synonyms

Synonyms Comment Organism
DAPDC
-
 Helicobacter pylori
meso-diaminopimelate decarboxylase
-
 Helicobacter pylori

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.07
-
 meso-2,6-diaminoheptanedioate mutant enzyme I148D, in 100 mM Tris, pH 8.0 Helicobacter pylori
0.07
-
 meso-2,6-diaminoheptanedioate mutant enzyme I148G, in 100 mM Tris, pH 8.0 Helicobacter pylori
0.28
-
 meso-2,6-diaminoheptanedioate mutant enzyme I148K, in 100 mM Tris, pH 8.0 Helicobacter pylori
0.33
-
 meso-2,6-diaminoheptanedioate mutant enzyme I148A, in 100 mM Tris, pH 8.0 Helicobacter pylori
0.38
-
 meso-2,6-diaminoheptanedioate mutant enzyme I148L, in 100 mM Tris, pH 8.0 Helicobacter pylori
0.68
-
 meso-2,6-diaminoheptanedioate mutant enzyme I148F, in 100 mM Tris, pH 8.0 Helicobacter pylori
15.65
-
 meso-2,6-diaminoheptanedioate wild type enzyme, in 100 mM Tris, pH 8.0 Helicobacter pylori

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Helicobacter pylori