Cloned (Comment) | Organism |
---|---|
mutated ODC is in vitro transcribed and translated in reticulocyte lysate, expression of His-tagged ODC fused to the human dihydrofolate reductase, DHFR, in Escherichia coli | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
A457W | site-directed mutagenesis, the mutation does not stabilize ODC | Mus musculus |
C441A | construction of a duplicated cODC, from fusing ODC to a tandem cODC-cODCC441A at its C-terminus, with the first ODC copy being wild-type including a wild-type Cys441, and the distal C-terminal copy of cODC carrying mutation C441A | Mus musculus |
additional information | construction of several truncated enzyme mutants, mutant protein stabilities, overview | Mus musculus |
S456A | site-directed mutagenesis, the mutation does not stabilize ODC | Mus musculus |
General Stability | Organism |
---|---|
mouse ODC is quickly degraded by the 26S proteasome in mammalian and fungal cells | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mus musculus | mouse ODC is quickly degraded by the 26S proteasome in an ubiquitin-dependent manner in mammalian and fungal cells. Within cODC, Cys441 functions as a proteasome association element, while the C-terminal end of cODC initiates entry into the proteasome | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | P00860 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | mouse ODC is quickly degraded by the 26S proteasome in an ubiquitin-dependent manner in mammalian and fungal cells. Within cODC, Cys441 functions as a proteasome association element, while the C-terminal end of cODC initiates entry into the proteasome | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ODC | - |
Mus musculus |