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Literature summary for 4.1.1.15 extracted from
- Enhanced production of recombinant Escherichia coli glutamate decarboxylase through optimization of induction strategy and addition of pyridoxine (2015), Biores. Technol., 198, 63-69 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene gad, recombinant lactose-induced overexpression of enzyme GAD in Escherichia coli strain BL21(DE3) | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | enhanced production of recombinant Escherichia coli glutamate decarboxylase through optimization of induction strategy and addition of pyridoxine, different induction strategies are investigated, induction is optimal when the temperature is maintained at 30°C, the inducer lactose is fed at a rate of 0.2 g/l/h, and protein expression is induced when the cell density (OD600) reaches 50. Under these conditions, the GAD activity of 1273.8 U/ml is achieved. The supplementing the medium with 2 mM pyridoxine hydrochloride (PN), a cheap and stable PLP precursor, at the initiation of protein expression, and then again 10 h later, results in very high GAD activity of 3193.4 U/ml. Fed-batch cultivation in a 3.6-L fermentor at 37°C and pH 7.0 | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate | Escherichia coli | - |
4-aminobutanoate + CO2 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P69910 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate | - |
Escherichia coli | 4-aminobutanoate + CO2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GAD | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.8 | - |
assay at | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | dependent on | Escherichia coli | |
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