Cloned (Comment) | Organism |
---|---|
expression in Sacchaormyces cerevisiae, His-tag | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
N-terminally truncated mutants of both isoform GAD65 and GAD67. GAD67 shows a thethered loop covering the active site, providing a catalytic environment that sustains 4-aminobutanoate production. In isoform GAD65, the same catalytic loop is inherently mobile promoting a side reaction that results in cofactor release and enzyme autoinactivation | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | crystallization data of N-terminally truncated isoform GAD67, expressing amino acids 90-594, catalytically active, and of N-terminally truncated isoform GAD65 expressing amino acids 84-585 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | isoform GAD65 undergoes a side reaction yielding pyridoxamine 5-phosphate, succinic semialdehyde and inactive apo enzyme | Homo sapiens | ? | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Homo sapiens |